7RCV

High-resolution structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803

7RCV の概要

• エントリーDOI: 10.2210/pdb7rcv/pdb
• EMDBエントリー: 24407
• 分子名称: Photosystem II protein D1 2, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (38 entities in total)
• 機能のキーワード: photosystem ii, cyanobacteria, synechocystis 6803, mesophile, cyanoq, photosynthesis
• 由来する生物種: Synechocystis sp. (strain PCC 6803 / Kazusa); 詳細
• タンパク質・核酸の鎖数: 42
• 化学式量合計: 808199.57

構造登録者

Gisriel, C.J.,Brudvig, G.W. (登録日: 2021-07-08, 公開日: 2021-12-29, 最終更新日: 2024-10-23)

主引用文献

Gisriel, C.J.,Wang, J.,Liu, J.,Flesher, D.A.,Reiss, K.M.,Huang, H.L.,Yang, K.R.,Armstrong, W.H.,Gunner, M.R.,Batista, V.S.,Debus, R.J.,Brudvig, G.W.
High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803.
Proc.Natl.Acad.Sci.USA, 119:-, 2022

PubMed Abstract: Photosystem II (PSII) enables global-scale, light-driven water oxidation. Genetic manipulation of PSII from the mesophilic cyanobacterium sp. PCC 6803 has provided insights into the mechanism of water oxidation; however, the lack of a high-resolution structure of oxygen-evolving PSII from this organism has limited the interpretation of biophysical data to models based on structures of thermophilic cyanobacterial PSII. Here, we report the cryo-electron microscopy structure of PSII from sp. PCC 6803 at 1.93-Å resolution. A number of differences are observed relative to thermophilic PSII structures, including the following: the extrinsic subunit PsbQ is maintained, the C terminus of the D1 subunit is flexible, some waters near the active site are partially occupied, and differences in the PsbV subunit block the Large (O1) water channel. These features strongly influence the structural picture of PSII, especially as it pertains to the mechanism of water oxidation.

PubMed: 34937700
DOI: 10.1073/pnas.2116765118

実験手法

ELECTRON MICROSCOPY (2.01 Å)