7RCD

Second stage reengineered variant of I-OnuI targeting human PD1 gene with activity enhancing substitutions

7RCD の概要

• エントリーDOI: 10.2210/pdb7rcd/pdb
• 分子名称: I-OnuI_e-hPD1-c, DNA (26-MER), CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: meganuclease, gene editing, protein engineering, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Synthetic construct; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 51054.76

構造登録者

Ubilla-Rodriguez, N.C.,Werther, R.A.,Stoddard, B.L. (登録日: 2021-07-07, 公開日: 2022-06-29, 最終更新日: 2023-10-18)

• 主引用文献: Werther, R.A.,Ubilla-Rodriguez, N.C.,Smiley, A.T.,Havens, K.,Lambert, A.R.,Stoddard, B.L.; Characterization of the stepwise engineering and optimization of a retargeted DNA binding protein and gene-editing meganuclease; To Be Published,

実験手法

X-RAY DIFFRACTION (2.448 Å)