7RC4

Aeronamide N-methyltransferase, AerE (D141A)

7RC4 の概要

• エントリーDOI: 10.2210/pdb7rc4/pdb
• 分子名称: Methyltransferase family protein, S-ADENOSYL-L-HOMOCYSTEINE, SODIUM ION, ... (7 entities in total)
• 機能のキーワード: sam-dependent, peptide cytotoxin, proteusin, transferase
• 由来する生物種: Microvirgula aerodenitrificans DSM 15089
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42910.55

構造登録者

Cogan, D.P.,Reyes, R.,Nair, S.K. (登録日: 2021-07-07, 公開日: 2022-03-30, 最終更新日: 2023-10-18)

主引用文献

Cogan, D.P.,Bhushan, A.,Reyes, R.,Zhu, L.,Piel, J.,Nair, S.K.
Structure and mechanism for iterative amide N -methylation in the biosynthesis of channel-forming peptide cytotoxins.
Proc.Natl.Acad.Sci.USA, 119:e2116578119-e2116578119, 2022

PubMed Abstract: SignificanceThe channel-forming proteusins are bacterial helical peptides that allow permeation of positively charged ions to influence membrane potential and cellular physiology. We biochemically characterize the effect of two critical posttranslational modifications on the secondary structure of the peptide substrate. We determine how a methyl group can be added to the side chains of D-Asn residues in a peptide substrate and show how flanking residues influence selectivity. These studies should foster the development of small-molecule peptide ion channels as therapeutics.

PubMed: 35316135
DOI: 10.1073/pnas.2116578119

実験手法

X-RAY DIFFRACTION (1.66 Å)