7R5A

Vibrio cholera ParD2:ParE2 antitoxin:toxin complex

7R5A の概要

• エントリーDOI: 10.2210/pdb7r5a/pdb
• 分子名称: Toxin, Antitoxin ParD (2 entities in total)
• 機能のキーワード: prokaryotic toxin:antitoxin system, intrinsically disordered proteins, rhh protein, dna binding protein, transcriptional repressor, antitoxin, toxin, gyrase-poison, vibrio cholerae
• 由来する生物種: Vibrio cholerae O1 biovar El Tor str. N16961; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 39353.56

構造登録者

Garcia-Rodriguez, G.,Loris, R. (登録日: 2022-02-10, 公開日: 2022-04-27, 最終更新日: 2024-01-31)

主引用文献

Garcia-Rodriguez, G.,Girardin, Y.,Kumar Singh, R.,Volkov, A.N.,Van Dyck, J.,Muruganandam, G.,Sobott, F.,Charlier, D.,Loris, R.
Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module.
Sci Adv, 10:eadj2403-eadj2403, 2024

PubMed Abstract: The family of toxin-antitoxin (TA) operons is ubiquitous in bacterial genomes and, in , is an essential component to maintain the presence of chromosome II. Here, we show that transcription of the (Vc) operon is regulated in a toxin:antitoxin ratio-dependent manner using a molecular mechanism distinct from other type II TA systems. The repressor of the operon is identified as an assembly with a 6:2 stoichiometry with three interacting ParD2 dimers bridged by two ParE2 monomers. This assembly docks to a three-site operator containing 5'- GGTA-3' motifs. Saturation of this TA complex with ParE2 toxin results in disruption of the interface between ParD2 dimers and the formation of a TA complex of 2:2 stoichiometry. The latter is operator binding-incompetent as it is incompatible with the required spacing of the ParD2 dimers on the operator.

PubMed: 38181072
DOI: 10.1126/sciadv.adj2403

実験手法

X-RAY DIFFRACTION (2.95 Å)