7R1E

Mosquitocidal Cry11Ba determined at pH 10.4 from naturally-occurring nanocrystals by Serial femtosecond crystallography

7R1E の概要

• エントリーDOI: 10.2210/pdb7r1e/pdb
• 分子名称: Pesticidal crystal protein Cry11Ba, GLYCEROL (3 entities in total)
• 機能のキーワード: naturally-occurring crystals mosquitocidal toxin serial femtosecond crystallography, toxin
• 由来する生物種: Bacillus thuringiensis serovar jegathesan
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 163009.44

構造登録者

Colletier, J.-P.,Sawaya, M.R.,Schibrowsky, N.A.,Cascio, D.,Rodriguez, J.A. (登録日: 2022-02-02, 公開日: 2022-07-27, 最終更新日: 2024-11-13)

主引用文献

Tetreau, G.,Sawaya, M.R.,De Zitter, E.,Andreeva, E.A.,Banneville, A.S.,Schibrowsky, N.A.,Coquelle, N.,Brewster, A.S.,Grunbein, M.L.,Kovacs, G.N.,Hunter, M.S.,Kloos, M.,Sierra, R.G.,Schiro, G.,Qiao, P.,Stricker, M.,Bideshi, D.,Young, I.D.,Zala, N.,Engilberge, S.,Gorel, A.,Signor, L.,Teulon, J.M.,Hilpert, M.,Foucar, L.,Bielecki, J.,Bean, R.,de Wijn, R.,Sato, T.,Kirkwood, H.,Letrun, R.,Batyuk, A.,Snigireva, I.,Fenel, D.,Schubert, R.,Canfield, E.J.,Alba, M.M.,Laporte, F.,Despres, L.,Bacia, M.,Roux, A.,Chapelle, C.,Riobe, F.,Maury, O.,Ling, W.L.,Boutet, S.,Mancuso, A.,Gutsche, I.,Girard, E.,Barends, T.R.M.,Pellequer, J.L.,Park, H.W.,Laganowsky, A.D.,Rodriguez, J.,Burghammer, M.,Shoeman, R.L.,Doak, R.B.,Weik, M.,Sauter, N.K.,Federici, B.,Cascio, D.,Schlichting, I.,Colletier, J.P.
De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals.
Nat Commun, 13:4376-4376, 2022

PubMed Abstract: Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.

PubMed: 35902572
DOI: 10.1038/s41467-022-31746-x

実験手法

X-RAY DIFFRACTION (2.65 Å)