7R0U

Structure of a cytosolic sulfotransferase of Anopheles gambiae (AGAP001425) in complex with 3'-phosphoadenosine 5-phosphate and vanillin.

7R0U の概要

• エントリーDOI: 10.2210/pdb7r0u/pdb
• 分子名称: AGAP001425-PA, 4-hydroxy-3-methoxybenzaldehyde, ADENOSINE-3'-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: anopheles gambiae, cytosolic sulfotransferase, sulfation, vanillin, 3'-phosphoadenosine 5-phosphate., transferase
• 由来する生物種: Anopheles gambiae (African malaria mosquito)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 87075.09

構造登録者

Esposito Verza, A.,Miggiano, R.,Rizzi, R.,Rossi, F. (登録日: 2022-02-02, 公開日: 2022-08-10, 最終更新日: 2024-01-31)

主引用文献

Esposito Verza, A.,Miggiano, R.,Lombardo, F.,Fiorillo, C.,Arca, B.,Purghe, B.,Del Grosso, E.,Galli, U.,Rizzi, M.,Rossi, F.
Biochemical and structural analysis of a cytosolic sulfotransferase of the malaria vector Anopheles gambiae overexpressed in the reproductive tissues.
Curr Res Struct Biol, 4:246-255, 2022

PubMed Abstract: The temporary or permanent chemical modification of biomolecules is a crucial aspect in the physiology of all living species. However, while some modules are well characterised also in insects, others did not receive the same attention. This holds true for sulfo-conjugation that is catalysed by cytosolic sulfotransferases (SULT), a central component of the metabolism of endogenous low molecular weight molecules and xenobiotics. In particular, limited information is available about the functional roles of the mosquito predicted enzymes annotated as SULTs in genomic databases. The herein described research is the first example of a biochemical and structural study of a SULT of a mosquito species, in general, and of the malaria vector in particular. We confirmed that the AGAP001425 transcript displays a peculiar expression pattern that is suggestive of a possible involvement in modulating the mosquito reproductive tissues physiology, a fact that could raise attention on the enzyme as a potential target for insect-containment strategies. The crystal structures of the enzyme in alternative ligand-bound states revealed elements distinguishing SULT-001425 from other characterized SULTs, including a peculiar conformational plasticity of a discrete region that shields the catalytic cleft and that could play a main role in the dynamics of the reaction and in the substrate selectivity of the enzyme. Along with further biochemical studies, our structural investigations could provide a framework for the discovery of small-molecule inhibitors to assess the effect of interfering with SULT-001425-mediated catalysis at the organismal level.

PubMed: 35941867
DOI: 10.1016/j.crstbi.2022.07.001

実験手法

X-RAY DIFFRACTION (2.5 Å)