7QZ2

Crystal structure of GacS D1 domain in complex with BeF3-

7QZ2 の概要

• エントリーDOI: 10.2210/pdb7qz2/pdb
• 関連するPDBエントリー: 6ZL8
• 分子名称: Histidine kinase, CADMIUM ION, BERYLLIUM TRIFLUORIDE ION, ... (5 entities in total)
• 機能のキーワード: transmitter domain histidine kinase pseudomonas aeruginosa, signaling protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35089.06

構造登録者

Fadel, F.,Bassim, V.,Botzanowski, T.,Francis, V.I.,Legrand, P.,Porter, S.L.,Bourne, Y.,Cianferani, S.,Vincent, F. (登録日: 2022-01-30, 公開日: 2022-07-06, 最終更新日: 2024-02-07)

主引用文献

Fadel, F.,Bassim, V.,Francis, V.I.,Porter, S.L.,Botzanowski, T.,Legrand, P.,Perez, M.M.,Bourne, Y.,Cianferani, S.,Vincent, F.
Insights into the atypical autokinase activity of the Pseudomonas aeruginosa GacS histidine kinase and its interaction with RetS.
Structure, 30:1285-1297.e5, 2022

PubMed Abstract: Virulence in Pseudomonas aeruginosa (PA) depends on complex regulatory networks, involving phosphorelay systems based on two-component systems (TCSs). The GacS/GacA TCS is a master regulator of biofilm formation, swarming motility, and virulence. GacS is a membrane-associated unorthodox histidine kinase (HK) whose phosphorelay signaling pathway is inhibited by the RetS hybrid HK. Here we provide structural and functional insights into the interaction of GacS with RetS. The structure of the GacS-HAMP-H1 cytoplasmic regions reveals an unusually elongated homodimer marked by a 135 Å long helical bundle formed by the HAMP, the signaling helix (S helix) and the DHp subdomain. The HAMP and S helix regions are essential for GacS signaling and contribute to the GacS/RetS binding interface. The structure of the GacS D1 domain together with the discovery of an unidentified functional ND domain, essential for GacS full autokinase activity, unveils signature motifs in GacS required for its atypical autokinase mechanism.

PubMed: 35767996
DOI: 10.1016/j.str.2022.06.002

実験手法

X-RAY DIFFRACTION (1.87 Å)