7QYF

Structure of the transaminase PluriZyme variant (TR2E2)

7QYF の概要

• エントリーDOI: 10.2210/pdb7qyf/pdb
• 分子名称: Aminotransferase TR2 (1 entity in total)
• 機能のキーワード: transaminase, hydrolase
• 由来する生物種: Acidihalobacter
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 206634.67

構造登録者

Roda, S.,Fernandez-Lopez, L.,Benedens, M.,Bollinger, A.,Thies, S.,Schumacher, J.,Coscolin, C.,Kazemi, M.,Santiago, G.,Gertzen, C.G.,Gonzalez-Alfonso, J.,Plou, F.J.,Jaeger, K.E.,Smits, S.H.,Ferrer, M.,Guallar, V. (登録日: 2022-01-28, 公開日: 2023-07-26, 最終更新日: 2026-03-04)

主引用文献

Roda, S.,Fernandez-Lopez, L.,Benedens, M.,Bollinger, A.,Thies, S.,Schumacher, J.,Coscolin, C.,Kazemi, M.,Santiago, G.,Gertzen, C.G.W.,Gonzalez-Alfonso, J.L.,Plou, F.J.,Jaeger, K.E.,Smits, S.H.J.,Ferrer, M.,Guallar, V.
A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.
Angew Chem Int Ed Engl, 61:e202207344-, 2022

PubMed Abstract: Engineering dual-function single polypeptide catalysts with two abiotic or biotic catalytic entities (or combinations of both) supporting cascade reactions is becoming an important area of enzyme engineering and catalysis. Herein we present the development of a PluriZyme, TR E , with efficient native transaminase (k : 69.49±1.77 min ) and artificial esterase (k : 3908-0.41 min ) activities integrated into a single scaffold, and evaluate its utility in a cascade reaction. TR E (pH : 8.0-9.5; T : 60-65 °C) efficiently converts methyl 3-oxo-4-(2,4,5-trifluorophenyl)butanoate into 3-(R)-amino-4-(2,4,5-trifluorophenyl)butanoic acid, a crucial intermediate for the synthesis of antidiabetic drugs. The reaction proceeds through the conversion of the β-keto ester into the β-keto acid at the hydrolytic site and subsequently into the β-amino acid (e.e. >99 %) at the transaminase site. The catalytic power of the TR E PluriZyme was proven with a set of β-keto esters, demonstrating the potential of such designs to address bioinspired cascade reactions.

PubMed: 35734849
DOI: 10.1002/anie.202207344

実験手法

X-RAY DIFFRACTION (3.3 Å)