7QWP

CryoEM structure of bacterial transcription close complex (RPc)

7QWP の概要

• エントリーDOI: 10.2210/pdb7qwp/pdb
• EMDBエントリー: 14190
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (7 entities in total)
• 機能のキーワード: cryo em, rna polymerase, transcription close complex, sigma54, transcription
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 484549.36

構造登録者

Ye, F.Z.,Zhang, X.D. (登録日: 2022-01-25, 公開日: 2022-11-09, 最終更新日: 2024-07-17)

主引用文献

Ye, F.,Gao, F.,Liu, X.,Buck, M.,Zhang, X.
Mechanisms of DNA opening revealed in AAA+ transcription complex structures.
Sci Adv, 8:eadd3479-eadd3479, 2022

PubMed Abstract: Gene transcription is carried out by RNA polymerase (RNAP) and requires the conversion of the initial closed promoter complex, where DNA is double stranded, to a transcription-competent open promoter complex, where DNA is opened up. In bacteria, RNAP relies on σ factors for its promoter specificities. Using a special form of sigma factor (σ), which forms a stable closed complex and requires its activator that belongs to the AAA+ ATPases (ATPases associated with diverse cellular activities), we obtained cryo-electron microscopy structures of transcription initiation complexes that reveal a previously unidentified process of DNA melting opening. The σ amino terminus threads through the locally opened up DNA and then becomes enclosed by the AAA+ hexameric ring in the activator-bound intermediate complex. Our structures suggest how ATP hydrolysis by the AAA+ activator could remove the σ inhibition while helping to open up DNA, using σ amino-terminal peptide as a pry bar.

PubMed: 36542713
DOI: 10.1126/sciadv.add3479

実験手法

ELECTRON MICROSCOPY (3.4 Å)