7QW9

Cryo-EM structure of coxsackievirus A6 mature virion

7QW9 の概要

• エントリーDOI: 10.2210/pdb7qw9/pdb
• EMDBエントリー: 14186
• 分子名称: Capsid protein VP1, Capsid protein VP2, Capsid protein VP3, ... (6 entities in total)
• 機能のキーワード: enterovirus, coxsackievirus a6, virion, native, capsid, cryo-em, virus
• 由来する生物種: Coxsackievirus A6; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 95771.38

構造登録者

Buttner, C.R.,Spurny, R.,Fuzik, T.,Plevka, P. (登録日: 2022-01-25, 公開日: 2022-09-07, 最終更新日: 2024-07-17)

主引用文献

Buttner, C.R.,Spurny, R.,Fuzik, T.,Plevka, P.
Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion.
Commun Biol, 5:898-898, 2022

PubMed Abstract: Coxsackievirus A6 (CV-A6) has recently overtaken enterovirus A71 and CV-A16 as the primary causative agent of hand, foot, and mouth disease worldwide. Virions of CV-A6 were not identified in previous structural studies, and it was speculated that the virus is unique among enteroviruses in using altered particles with expanded capsids to infect cells. In contrast, the virions of other enteroviruses are required for infection. Here we used cryo-electron microscopy (cryo-EM) to determine the structures of the CV-A6 virion, altered particle, and empty capsid. We show that the CV-A6 virion has features characteristic of virions of other enteroviruses, including a compact capsid, VP4 attached to the inner capsid surface, and fatty acid-like molecules occupying the hydrophobic pockets in VP1 subunits. Furthermore, we found that in a purified sample of CV-A6, the ratio of infectious units to virions is 1 to 500. Therefore, it is likely that virions of CV-A6 initiate infection, like those of other enteroviruses. Our results provide evidence that future vaccines against CV-A6 should target its virions instead of the antigenically distinct altered particles. Furthermore, the structure of the virion provides the basis for the rational development of capsid-binding inhibitors that block the genome release of CV-A6.

PubMed: 36056184
DOI: 10.1038/s42003-022-03863-2

実験手法

ELECTRON MICROSCOPY (2.68 Å)