7QV1

Bacillus subtilis collided disome (Leading 70S)

これはPDB形式変換不可エントリーです。

7QV1 の概要

• エントリーDOI: 10.2210/pdb7qv1/pdb
• 関連するPDBエントリー: 7QV1 7QV2 7QV3
• EMDBエントリー: 14156 14157 14157 14158 14159 14160 14161 14162 14163 14164 14165 14166
• 分子名称: 50S ribosomal protein L32, 50S ribosomal protein L4, 50S ribosomal protein L5, ... (53 entities in total)
• 機能のキーワード: collision, muts2, disome, rqc, ssra, ribosomal collision, mrna endonuclease, ribosome
• 由来する生物種: Bacillus subtilis subsp. subtilis str. 168; 詳細
• タンパク質・核酸の鎖数: 53
• 化学式量合計: 2131509.94

構造登録者

Filbeck, S.,Pfeffer, S. (登録日: 2022-01-19, 公開日: 2022-03-09, 最終更新日: 2022-03-30)

主引用文献

Cerullo, F.,Filbeck, S.,Patil, P.R.,Hung, H.C.,Xu, H.,Vornberger, J.,Hofer, F.W.,Schmitt, J.,Kramer, G.,Bukau, B.,Hofmann, K.,Pfeffer, S.,Joazeiro, C.A.P.
Bacterial ribosome collision sensing by a MutS DNA repair ATPase paralogue.
Nature, 603:509-514, 2022

PubMed Abstract: Ribosome stalling during translation is detrimental to cellular fitness, but how this is sensed and elicits recycling of ribosomal subunits and quality control of associated mRNA and incomplete nascent chains is poorly understood. Here we uncover Bacillus subtilis MutS2, a member of the conserved MutS family of ATPases that function in DNA mismatch repair, as an unexpected ribosome-binding protein with an essential function in translational quality control. Cryo-electron microscopy analysis of affinity-purified native complexes shows that MutS2 functions in sensing collisions between stalled and translating ribosomes and suggests how ribosome collisions can serve as platforms to deploy downstream processes: MutS2 has an RNA endonuclease small MutS-related (SMR) domain, as well as an ATPase/clamp domain that is properly positioned to promote ribosomal subunit dissociation, which is a requirement both for ribosome recycling and for initiation of ribosome-associated protein quality control (RQC). Accordingly, MutS2 promotes nascent chain modification with alanine-tail degrons-an early step in RQC-in an ATPase domain-dependent manner. The relevance of these observations is underscored by evidence of strong co-occurrence of MutS2 and RQC genes across bacterial phyla. Overall, the findings demonstrate a deeply conserved role for ribosome collisions in mounting a complex response to the interruption of translation within open reading frames.

PubMed: 35264791
DOI: 10.1038/s41586-022-04487-6

実験手法

ELECTRON MICROSCOPY (3.5 Å)