7QUJ

Structure of NsNEPS2, a 7S-cis-trans nepetalactone synthase

7QUJ の概要

• エントリーDOI: 10.2210/pdb7quj/pdb
• 分子名称: NsNEPS2, NICOTINAMIDE-ADENINE-DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: sdr, cyclisation, oxidation, nad+, biosynthetic protein
• 由来する生物種: Nepeta sibirica
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 122246.45

構造登録者

Hernandez Lozada, N.J.,Hong, B.,Wood, J.C.,Caputi, L.,Basquin, J.,Chuang, L.,Kunert, M.,Rodriguez Lopez, C.R.,Langley, C.,Zhao, D.,Buell, C.R.,Lichman, B.R.,O'Connor, S.E. (登録日: 2022-01-18, 公開日: 2022-12-28, 最終更新日: 2024-01-31)

主引用文献

Hernandez Lozada, N.J.,Hong, B.,Wood, J.C.,Caputi, L.,Basquin, J.,Chuang, L.,Kunert, M.,Rodriguez Lopez, C.E.,Langley, C.,Zhao, D.,Buell, C.R.,Lichman, B.R.,O'Connor, S.E.
Biocatalytic routes to stereo-divergent iridoids.
Nat Commun, 13:4718-4718, 2022

PubMed Abstract: Thousands of natural products are derived from the fused cyclopentane-pyran molecular scaffold nepetalactol. These natural products are used in an enormous range of applications that span the agricultural and medical industries. For example, nepetalactone, the oxidized derivative of nepetalactol, is known for its cat attractant properties as well as potential as an insect repellent. Most of these naturally occurring nepetalactol-derived compounds arise from only two out of the eight possible stereoisomers, 7S-cis-trans and 7R-cis-cis nepetalactols. Here we use a combination of naturally occurring and engineered enzymes to produce seven of the eight possible nepetalactol or nepetalactone stereoisomers. These enzymes open the possibilities for biocatalytic production of a broader range of iridoids, providing a versatile system for the diversification of this important natural product scaffold.

PubMed: 35953485
DOI: 10.1038/s41467-022-32414-w

実験手法

X-RAY DIFFRACTION (1.85 Å)