7QSV

L8-complex forming RubisCO derived from ancestral sequence reconstruction of the last common ancestor of Form I'' and Form I RubisCOs

7QSV の概要

• エントリーDOI: 10.2210/pdb7qsv/pdb
• 分子名称: RubisCO large subunit, 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: ribulose 1, 5-bisphosphate carboxylase/oxydase, rubisco, lyase
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 410920.30

構造登録者

Zarzycki, J.,Schulz, L.,Erb, T.J.,Hochberg, G.K.A. (登録日: 2022-01-14, 公開日: 2022-10-12, 最終更新日: 2024-01-31)

主引用文献

Schulz, L.,Guo, Z.,Zarzycki, J.,Steinchen, W.,Schuller, J.M.,Heimerl, T.,Prinz, S.,Mueller-Cajar, O.,Erb, T.J.,Hochberg, G.K.A.
Evolution of increased complexity and specificity at the dawn of form I Rubiscos.
Science, 378:155-160, 2022

PubMed Abstract: The evolution of ribulose-1,5-bisphosphate carboxylase/oxygenases (Rubiscos) that discriminate strongly between their substrate carbon dioxide and the undesired side substrate dioxygen was an important event for photosynthetic organisms adapting to an oxygenated environment. We use ancestral sequence reconstruction to recapitulate this event. We show that Rubisco increased its specificity and carboxylation efficiency through the gain of an accessory subunit before atmospheric oxygen was present. Using structural and biochemical approaches, we retrace how this subunit was gained and became essential. Our work illuminates the emergence of an adaptation to rising ambient oxygen levels, provides a template for investigating the function of interactions that have remained elusive because of their essentiality, and sheds light on the determinants of specificity in Rubisco.

PubMed: 36227987
DOI: 10.1126/science.abq1416

実験手法

X-RAY DIFFRACTION (2.1 Å)