7QST

Crystal structure of homing endonuclease-associated PhoVMA intein (C1A)

7QST の概要

• エントリーDOI: 10.2210/pdb7qst/pdb
• 分子名称: V-type ATP synthase subunit A, CADMIUM ION, NICKEL (II) ION, ... (4 entities in total)
• 機能のキーワード: intein, protein splicing, endonuclease, hydrolase
• 由来する生物種: Pyrococcus horikoshii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42984.07

構造登録者

Beyer, H.M.,Iwai, H. (登録日: 2022-01-14, 公開日: 2022-03-30, 最終更新日: 2024-01-31)

主引用文献

Beyer, H.M.,Iwai, H.
Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins.
Front Mol Biosci, 9:855511-855511, 2022

PubMed Abstract: Inteins catalyze their removal from a host protein through protein splicing. Inteins that contain an additional site-specific endonuclease domain display genetic mobility via a process termed "homing" and thereby act as selfish DNA elements. We elucidated the crystal structures of two archaeal inteins associated with an active or inactive homing endonuclease domain. This analysis illustrated structural diversity in the accessory domains (ACDs) associated with the homing endonuclease domain. To augment homing endonucleases with highly specific DNA cleaving activity using the intein scaffold, we engineered the ACDs and characterized their homing site recognition. Protein engineering of the ACDs in the inteins illuminated a possible strategy for how inteins could avoid their extinction but spread via the acquisition of a diverse accessory domain.

PubMed: 35372505
DOI: 10.3389/fmolb.2022.855511

実験手法

X-RAY DIFFRACTION (2.49 Å)