7QP8

Crystal structure of Vibrio alkaline phosphatase with bound HEPES

7QP8 の概要

• エントリーDOI: 10.2210/pdb7qp8/pdb
• 分子名称: Alkaline phosphatase, ZINC ION, MAGNESIUM ION, ... (8 entities in total)
• 機能のキーワード: phosphatase, hepes, hydrolase
• 由来する生物種: Vibrio sp. G15-21
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 120658.09

構造登録者

Markusson, S.,Hjorleifsson, J.G.,Kursula, P.,Asgeirsson, B. (登録日: 2022-01-03, 公開日: 2022-11-02, 最終更新日: 2024-01-31)

主引用文献

Markusson, S.,Hjorleifsson, J.G.,Kursula, P.,Asgeirsson, B.
Structural Characterization of Functionally Important Chloride Binding Sites in the Marine Vibrio Alkaline Phosphatase.
Biochemistry, 61:2248-2260, 2022

PubMed Abstract: Enzyme stability and function can be affected by various environmental factors, such as temperature, pH, and ionic strength. Enzymes that are located outside the relatively unchanging environment of the cytosol, such as those residing in the periplasmic space of bacteria or extracellularly secreted, are challenged by more fluctuations in the aqueous medium. Bacterial alkaline phosphatases (APs) are generally affected by ionic strength of the medium, but this varies substantially between species. An AP from the marine bacterium (VAP) shows complex pH-dependent activation and stabilization in the 0-1.0 M range of halogen salts and has been hypothesized to specifically bind chloride anions. Here, using X-ray crystallography and anomalous scattering, we have located two chloride binding sites in the structure of VAP, one in the active site and another one at a peripheral site. Further characterization of the binding sites using site-directed mutagenesis and small-angle X-ray scattering showed that upon binding of chloride to the peripheral site, structural dynamics decreased locally, resulting in thermal stabilization of the VAP active conformation. Binding of the chloride ion in the active site did not displace the bound inorganic phosphate product, but it may promote product release by facilitating rotational stabilization of the substrate-binding Arg129. Overall, these results reveal the complex nature and dynamics of chloride binding to enzymes through long-range modulation of electronic potential in the vicinity of the active site, resulting in increased catalytic efficiency and stability.

PubMed: 36194497
DOI: 10.1021/acs.biochem.2c00438

実験手法

X-RAY DIFFRACTION (1.7 Å)