7QP2

1-deazaguanosine modified-RNA Sarcin Ricin Loop

7QP2 の概要

• エントリーDOI: 10.2210/pdb7qp2/pdb
• 分子名称: RNA (27-MER), GLYCEROL (3 entities in total)
• 機能のキーワード: modified-rna, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8835.36

構造登録者

Ennifar, E.,Micura, R.,Bereiter, R.,Renard, E.,Kreutz, C. (登録日: 2021-12-30, 公開日: 2022-07-20, 最終更新日: 2024-01-31)

主引用文献

Bereiter, R.,Renard, E.,Breuker, K.,Kreutz, C.,Ennifar, E.,Micura, R.
1-Deazaguanosine-Modified RNA: The Missing Piece for Functional RNA Atomic Mutagenesis.
J.Am.Chem.Soc., 144:10344-10352, 2022

PubMed Abstract: Atomic mutagenesis is the key to advance our understanding of RNA recognition and RNA catalysis. To this end, deazanucleosides are utilized to evaluate the participation of specific atoms in these processes. One of the remaining challenges is access to RNA-containing 1-deazaguanosine (cG). Here, we present the synthesis of this nucleoside and its phosphoramidite, allowing first time access to cG-modified RNA. Thermodynamic analyses revealed the base pairing parameters for cG-modified RNA. Furthermore, by NMR spectroscopy, a cG-triggered switch of Watson-Crick into Hoogsteen pairing in HIV-2 TAR RNA was identified. Additionally, using X-ray structure analysis, a guanine-phosphate backbone interaction affecting RNA fold stability was characterized, and finally, the critical impact of an active-site guanine in twister ribozyme on the phosphodiester cleavage was revealed. Taken together, our study lays the synthetic basis for cG-modified RNA and demonstrates the power of the completed deazanucleoside toolbox for RNA atomic mutagenesis needed to achieve in-depth understanding of RNA recognition and catalysis.

PubMed: 35666572
DOI: 10.1021/jacs.2c01877

実験手法

X-RAY DIFFRACTION (0.9 Å)