7QOP
A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability
Summary for 7QOP
| Entry DOI | 10.2210/pdb7qop/pdb |
| Descriptor | Nitrile hydratase, Nitrile hydratase subunit beta, COBALT (II) ION, ... (6 entities in total) |
| Functional Keywords | heterotetramer, lyase |
| Biological source | Aeribacillus pallidus More |
| Total number of polymer chains | 2 |
| Total formula weight | 50341.82 |
| Authors | Van Wyk, J.C.,Cowan, D.A.,Danson, M.J.,Tsekoa, T.L.,Sayed, M.F.,Sewell, B.T. (deposition date: 2021-12-24, release date: 2023-01-18, Last modification date: 2024-11-06) |
| Primary citation | Van Wyk, J.C.,Sewell, B.T.,Danson, M.J.,Tsekoa, T.L.,Sayed, M.F.,Cowan, D.A. Engineering enhanced thermostability into the Geobacillus pallidus nitrile hydratase. Curr Res Struct Biol, 4:256-270, 2022 Cited by PubMed Abstract: Nitrile hydratases (NHases) are important biocatalysts for the enzymatic conversion of nitriles to industrially-important amides such as acrylamide and nicotinamide. Although thermostability in this enzyme class is generally low, there is not sufficient understanding of its basis for rational enzyme design. The gene expressing the Co-type NHase from the moderate thermophile, RAPc8 (NRRL B-59396), was subjected to random mutagenesis. Four mutants were selected that were 3 to 15-fold more thermostable than the wild-type NHase, resulting in a 3.4-7.6 kJ/mol increase in the activation energy of thermal inactivation at 63 °C. High resolution X-ray crystal structures (1.15-1.80 Å) were obtained of the wild-type and four mutant enzymes. Mutant 9E, with a resolution of 1.15 Å, is the highest resolution crystal structure obtained for a nitrile hydratase to date. Structural comparisons between the wild-type and mutant enzymes illustrated the importance of salt bridges and hydrogen bonds in enhancing NHase thermostability. These additional interactions variously improved thermostability by increased intra- and inter-subunit interactions, preventing cooperative unfolding of α-helices and stabilising loop regions. Some hydrogen bonds were mediated via a water molecule, specifically highlighting the significance of structured water molecules in protein thermostability. Although knowledge of the mutant structures makes it possible to rationalize their behaviour, it would have been challenging to predict in advance that these mutants would be stabilising. PubMed: 36106339DOI: 10.1016/j.crstbi.2022.07.002 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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