7QOD

Native structure of a small alarmone hydrolase (RelH) from Corynebacterium glutamicum

7QOD の概要

• エントリーDOI: 10.2210/pdb7qod/pdb
• 分子名称: Guanosine polyphosphate pyrophosphohydrolases/synthetases, MANGANESE (II) ION, TETRAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: hydrolase, alarmone, stress response, hd domain
• 由来する生物種: Corynebacterium glutamicum ATCC 13032
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45259.11

構造登録者

Bisiak, F.,Brodersen, D.E.,Chrenkova, A. (登録日: 2021-12-23, 公開日: 2022-07-13, 最終更新日: 2024-11-13)

主引用文献

Bisiak, F.,Chrenkova, A.,Zhang, S.D.,Pedersen, J.N.,Otzen, D.E.,Zhang, Y.E.,Brodersen, D.E.
Structural variations between small alarmone hydrolase dimers support different modes of regulation of the stringent response.
J.Biol.Chem., 298:102142-102142, 2022

PubMed Abstract: The bacterial stringent response involves wide-ranging metabolic reprogramming aimed at increasing long-term survivability during stress conditions. One of the hallmarks of the stringent response is the production of a set of modified nucleotides, known as alarmones, which affect a multitude of cellular pathways in diverse ways. Production and degradation of these molecules depend on the activity of enzymes from the RelA/SpoT homologous family, which come in both bifunctional (containing domains to both synthesize and hydrolyze alarmones) and monofunctional (consisting of only synthetase or hydrolase domain) variants, of which the structure, activity, and regulation of the bifunctional RelA/SpoT homologs have been studied most intensely. Despite playing an important role in guanosine nucleotide homeostasis in particular, mechanisms of regulation of the small alarmone hydrolases (SAHs) are still rather unclear. Here, we present crystal structures of SAH enzymes from Corynebacterium glutamicum (RelH) and Leptospira levettii (RelH) and show that while being highly similar, structural differences in substrate access and dimer conformations might be important for regulating their activity. We propose that a varied dimer form is a general property of the SAH family, based on current structural information as well as prediction models for this class of enzymes. Finally, subtle structural variations between monofunctional and bifunctional enzymes point to how these different classes of enzymes are regulated.

PubMed: 35714769
DOI: 10.1016/j.jbc.2022.102142

実験手法

X-RAY DIFFRACTION (1.85 Å)