7QIU

YsgA 23s RNA methyltransferase from Bacillus subtilis

7QIU の概要

• エントリーDOI: 10.2210/pdb7qiu/pdb
• 分子名称: rRNA methylase (2 entities in total)
• 機能のキーワード: 23s rna methyltransferase, transferase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58198.00

構造登録者

Labar, G.,Van Elder, D.,Roovers, M.,Droogmans, L. (登録日: 2021-12-16, 公開日: 2022-12-28, 最終更新日: 2024-02-07)

主引用文献

Roovers, M.,Labar, G.,Wolff, P.,Feller, A.,Van Elder, D.,Soin, R.,Gueydan, C.,Kruys, V.,Droogmans, L.
The Bacillus subtilis open reading frame ysgA encodes the SPOUT methyltransferase RlmP forming 2'- O -methylguanosine at position 2553 in the A-loop of 23S rRNA.
Rna, 28:1185-1196, 2022

PubMed Abstract: A previous bioinformatic analysis predicted that the open reading frame of encodes an RNA methyltransferase of the SPOUT superfamily. Here we show that YsgA is the 2'--methyltransferase that targets position G2553 ( numbering) of the A-loop of 23S rRNA. This was shown by a combination of biochemical and mass spectrometry approaches using both rRNA extracted from wild-type or cells and in vitro synthesized rRNA. When the target G2553 is mutated, YsgA is able to methylate the ribose of adenosine. However, it cannot methylate cytidine nor uridine. The enzyme modifies free 23S rRNA but not the fully assembled ribosome nor the 50S subunit, suggesting that the modification occurs early during ribosome biogenesis. Nevertheless, ribosome subunits assembly is unaffected in a mutant strain. The crystal structure of the recombinant YsgA protein, combined with mutagenesis data, outlined in this article highlights a typical SPOUT fold preceded by an L7Ae/L30 (eL8/eL30 in a new nomenclature) amino-terminal domain.

PubMed: 35710145
DOI: 10.1261/rna.079131.122

実験手法

X-RAY DIFFRACTION (1.877 Å)