7QCW

Apo-structure of serine hydroxymethyltransferase (PbzB) involved in benzobactin biosynthesis in P. chlororaphis subsp. piscium DSM 21509

7QCW の概要

• エントリーDOI: 10.2210/pdb7qcw/pdb
• 分子名称: Serine hydroxymethyltransferase (1 entity in total)
• 機能のキーワード: natural product biosynthesis, benzobactin, serine hydroxymethyltransferase, benzoxazolinate, biosynthetic protein
• 由来する生物種: Pseudomonas chlororaphis subsp. piscium
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 98914.42

構造登録者

Czech, L.,Bange, G. (登録日: 2021-11-25, 公開日: 2022-10-12, 最終更新日: 2024-01-31)

主引用文献

Shi, Y.M.,Crames, J.J.,Czech, L.,Bozhuyuk, K.A.J.,Shi, Y.N.,Hirschmann, M.,Lamberth, S.,Claus, P.,Paczia, N.,Ruckert, C.,Kalinowski, J.,Bange, G.,Bode, H.B.
Genome Mining Enabled by Biosynthetic Characterization Uncovers a Class of Benzoxazolinate-Containing Natural Products in Diverse Bacteria.
Angew.Chem.Int.Ed.Engl., 61:e202206106-e202206106, 2022

PubMed Abstract: Benzoxazolinate is a rare bis-heterocyclic moiety that interacts with proteins and DNA and confers extraordinary bioactivities on natural products, such as C-1027. However, the biosynthetic gene responsible for the key cyclization step of benzoxazolinate remains unclear. Herein, we show a putative acyl AMP-ligase responsible for the last cyclization step. We used the enzyme as a probe for genome mining and discovered that the orphan benzobactin gene cluster in entomopathogenic bacteria prevails across Proteobacteria and Firmicutes. It turns out that Pseudomonas chlororaphis produces various benzobactins, whose biosynthesis is highlighted by a synergistic effect of two unclustered genes encoding enzymes on boosting benzobactin production; the formation of non-proteinogenic 2-hydroxymethylserine by a serine hydroxymethyltransferase; and the types I and II NRPS architecture for structural diversity. Our findings reveal the biosynthetic potential of a widespread benzobactin gene cluster.

PubMed: 36198080
DOI: 10.1002/anie.202206106

実験手法

X-RAY DIFFRACTION (2.81 Å)