7QBZ

Crystal structure Cadmium translocating P-type ATPase

7QBZ の概要

• エントリーDOI: 10.2210/pdb7qbz/pdb
• 分子名称: Cadmium translocating P-type ATPase, TETRAFLUOROALUMINATE ION, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: p-type atpase pib-atpase transporter exporter, membrane protein
• 由来する生物種: Sulfitobacter sp. (strain NAS-14.1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 71781.51

構造登録者

Groenberg, C.,Hu, Q.,Wang, K.,Gourdon, P. (登録日: 2021-11-21, 公開日: 2022-03-23, 最終更新日: 2024-01-31)

主引用文献

Gronberg, C.,Hu, Q.,Mahato, D.R.,Longhin, E.,Salustros, N.,Duelli, A.,Lyu, P.,Bagenholm, V.,Eriksson, J.,Rao, K.U.,Henderson, D.I.,Meloni, G.,Andersson, M.,Croll, T.,Godaly, G.,Wang, K.,Gourdon, P.
Structure and ion-release mechanism of P IB-4 -type ATPases.
Elife, 10:-, 2021

PubMed Abstract: Transition metals, such as zinc, are essential micronutrients in all organisms, but also highly toxic in excessive amounts. Heavy-metal transporting P-type (P) ATPases are crucial for homeostasis, conferring cellular detoxification and redistribution through transport of these ions across cellular membranes. No structural information is available for the P-ATPases, the subclass with the broadest cargo scope, and hence even their topology remains elusive. Here, we present structures and complementary functional analyses of an archetypal P-ATPase, sCoaT from sp. NAS14-1. The data disclose the architecture, devoid of classical so-called heavy-metal-binding domains (HMBDs), and provide fundamentally new insights into the mechanism and diversity of heavy-metal transporters. We reveal several novel P-type ATPase features, including a dual role in heavy-metal release and as an internal counter ion of an invariant histidine. We also establish that the turnover of P-ATPases is potassium independent, contrasting to many other P-type ATPases. Combined with new inhibitory compounds, our results open up for efforts in for example drug discovery, since P-ATPases function as virulence factors in many pathogens.

PubMed: 34951590
DOI: 10.7554/eLife.73124

実験手法

X-RAY DIFFRACTION (3.25 Å)