7QB3

Solution structure of a lanthanide-binding DNA aptamer

7QB3 の概要

• エントリーDOI: 10.2210/pdb7qb3/pdb
• NMR情報: BMRB: 34685
• 分子名称: Lanthanide-binding aptamer, LUTETIUM (III) ION (2 entities in total)
• 機能のキーワード: aptamer, lanthanide, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8817.51

構造登録者

Andralojc, W.,Gdaniec, Z. (登録日: 2021-11-18, 公開日: 2021-12-01, 最終更新日: 2024-06-19)

主引用文献

Andralojc, W.,Wieruszewska, J.,Pasternak, K.,Gdaniec, Z.
Solution Structure of a Lanthanide-binding DNA Aptamer Determined Using High Quality pseudocontact shift restraints.
Chemistry, 28:e202202114-e202202114, 2022

PubMed Abstract: In this contribution we report the high-resolution NMR structure of a recently identified lanthanide-binding aptamer (LnA). We demonstrate that the rigid lanthanide binding by LnA allows for the measurement of anisotropic paramagnetic NMR restraints which to date remain largely inaccessible for nucleic acids. One type of such restraints - pseudocontact shifts (PCS) induced by four different paramagnetic lanthanides - was extensively used throughout the current structure determination study and the measured PCS turned out to be exceptionally well reproduced by the final aptamer structure. This finding opens the perspective for a broader application of paramagnetic effects in NMR studies of nucleic acids through the transplantation of the binding site found in LnA into other DNA/RNA systems.

PubMed: 36043489
DOI: 10.1002/chem.202202114

実験手法

SOLUTION NMR