7QA9

10bp DNA/DNA duplex

7QA9 の概要

• エントリーDOI: 10.2210/pdb7qa9/pdb
• NMR情報: BMRB: 34679
• 分子名称: DNA (5'-D(*CP*CP*AP*TP*TP*AP*TP*AP*GP*C)-3'), DNA (5'-D(*GP*CP*TP*AP*TP*AP*AP*TP*GP*G)-3') (2 entities in total)
• 機能のキーワード: duplex, 10bp, dna
• 由来する生物種: Octodon degus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6088.03

構造登録者

Li, Q.,Trajkovski, M.,Fan, C.,Chen, J.,Zhou, Y.,Lu, K.,Li, H.,Su, X.,Xi, Z.,Plavec, J.,Zhou, C. (登録日: 2021-11-16, 公開日: 2022-11-16, 最終更新日: 2024-06-19)

主引用文献

Li, Q.,Trajkovski, M.,Fan, C.,Chen, J.,Zhou, Y.,Lu, K.,Li, H.,Su, X.,Xi, Z.,Plavec, J.,Zhou, C.
4'-SCF 3 -Labeling Constitutes a Sensitive 19 F NMR Probe for Characterization of Interactions in the Minor Groove of DNA.
Angew.Chem.Int.Ed.Engl., 61:e202201848-e202201848, 2022

PubMed Abstract: Fluorinated nucleotides are invaluable for F NMR studies of nucleic acid structure and function. Here, we synthesized 4'-SCF -thymidine (T ) and incorporated it into DNA by means of solid-phase DNA synthesis. NMR studies showed that the 4'-SCF group exhibited a flexible orientation in the minor groove of DNA duplexes and was well accommodated by various higher order DNA structures. The three magnetically equivalent fluorine atoms in 4'-SCF -DNA constitute an isolated spin system, offering high F NMR sensitivity and excellent resolution of the positioning of T within various secondary and tertiary DNA structures. The high structural adaptability and high sensitivity of T make it a valuable F NMR probe for quantitatively distinguishing diverse DNA structures with single-nucleotide resolution and for monitoring the dynamics of interactions in the minor groove of double-stranded DNA.

PubMed: 36163470
DOI: 10.1002/anie.202201848

実験手法

SOLUTION NMR