7Q6O

Structure of WrbA from Yersinia pseudotuberculosis in C2221

7Q6O の概要

• エントリーDOI: 10.2210/pdb7q6o/pdb
• 分子名称: NAD(P)H dehydrogenase (quinone), CHLORIDE ION (3 entities in total)
• 機能のキーワード: nadh dehydrogenase, oxidoreductase, flavoprotein
• 由来する生物種: Yersinia pseudotuberculosis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49312.47

構造登録者

Gabrielsen, M.,Beckham, K.S.H.,Roe, A.J. (登録日: 2021-11-08, 公開日: 2022-08-03, 最終更新日: 2024-01-31)

主引用文献

Zambelloni, R.,Beckham, K.S.H.,Wu, H.J.,Elofsson, M.,Marquez, R.,Gabrielsen, M.,Roe, A.J.
Crystal structures of WrbA, a spurious target of the salicylidene acylhydrazide inhibitors of type III secretion in Gram-negative pathogens, and verification of improved specificity of next-generation compounds.
Microbiology (Reading, Engl.), 168:-, 2022

PubMed Abstract: The enterohemorrhagic pathotype is responsible for severe and dangerous infections in humans. Establishment of the infection requires colonization of the gastro-intestinal tract, which is dependent on the Type III Secretion System. The Type III Secretion System (T3SS) allows attachment of the pathogen to the mammalian host cell and cytoskeletal rearrangements within the host cell. Blocking the functionality of the T3SS is likely to reduce colonization and therefore limit the disease. This route offers an alternative to antibiotics, and problems with the development of antibiotics resistance. Salicylidene acylhydrazides have been shown to have an inhibitory effect on the T3SS in several pathogens. However, the main target of these compounds is still unclear. Past work has identified a number of putative protein targets of these compounds, one of which being WrbA. Whilst WrbA is considered an off-target interaction, this study presents the effect of the salicylidne acylhydrazide compounds on the activity of WrbA, along with crystal structures of WrbA from and serovar Typhimurium; the latter also containing parts of the compound in the structure. We also present data showing that the original compounds were unstable in acidic conditions, and that later compounds showed improved stability.

PubMed: 35829699
DOI: 10.1099/mic.0.001211

実験手法

X-RAY DIFFRACTION (1.99 Å)