7Q5A

Lanreotide nanotube

7Q5A の概要

• エントリーDOI: 10.2210/pdb7q5a/pdb
• EMDBエントリー: 13830
• 分子名称: Lanreotide (1 entity in total)
• 機能のキーワード: lanreotide, nanotube, assembly, hormone
• 由来する生物種: Homo sapiens
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 8786.71

構造登録者

Pieri, L.,Wang, F.,Arteni, A.A.,Bressanelli, S.,Egelman, E.H.,Paternostre, M. (登録日: 2021-11-03, 公開日: 2022-04-06, 最終更新日: 2024-11-13)

主引用文献

Pieri, L.,Wang, F.,Arteni, A.A.,Vos, M.,Winter, J.M.,Le Du, M.H.,Artzner, F.,Gobeaux, F.,Legrand, P.,Boulard, Y.,Bressanelli, S.,Egelman, E.H.,Paternostre, M.
Atomic structure of Lanreotide nanotubes revealed by cryo-EM.
Proc.Natl.Acad.Sci.USA, 119:-, 2022

PubMed Abstract: Functional and versatile nano- and microassemblies formed by biological molecules are found at all levels of life, from cell organelles to full organisms. Understanding the chemical and physicochemical determinants guiding the formation of these assemblies is crucial not only to understand the biological processes they carry out but also to mimic nature. Among the synthetic peptides forming well-defined nanostructures, the octapeptide Lanreotide has been considered one of the best characterized, in terms of both the atomic structure and its self-assembly process. In the present work, we determined the atomic structure of Lanreotide nanotubes at 2.5-Å resolution by cryoelectron microscopy (cryo-EM). Surprisingly, the asymmetric unit in the nanotube contains eight copies of the peptide, forming two tetramers. There are thus eight different environments for the peptide, and eight different conformations in the nanotube. The structure built from the cryo-EM map is strikingly different from the molecular model, largely based on X-ray fiber diffraction, proposed 20 y ago. Comparison of the nanotube with a crystal structure at 0.83-Å resolution of a Lanreotide derivative highlights the polymorphism for this peptide family. This work shows once again that higher-order assemblies formed by even well-characterized small peptides are very difficult to predict.

PubMed: 35042822
DOI: 10.1073/pnas.2120346119

実験手法

ELECTRON MICROSCOPY (2.46 Å)