7Q22

cryo iDPC-STEM structure recorded with CSA 2.0

7Q22 の概要

• エントリーDOI: 10.2210/pdb7q22/pdb
• EMDBエントリー: 13778
• 分子名称: Capsid protein, RNA (5'-R(P*GP*AP*A)-3') (2 entities in total)
• 機能のキーワード: tobacco mosaic virus, rna virus, virus
• 由来する生物種: Tobacco mosaic virus (strain vulgare); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18050.66

構造登録者

Sachse, C.,Leidl, M.L. (登録日: 2021-10-22, 公開日: 2022-09-21, 最終更新日: 2024-07-17)

主引用文献

Lazic, I.,Wirix, M.,Leidl, M.L.,de Haas, F.,Mann, D.,Beckers, M.,Pechnikova, E.V.,Muller-Caspary, K.,Egoavil, R.,Bosch, E.G.T.,Sachse, C.
Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution.
Nat.Methods, 19:1126-1136, 2022

PubMed Abstract: In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC-STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The micrographs show complete contrast transfer to high resolution and enable the cryo-EM structure determination for KLH at 6.5 Å resolution, as well as for TMV at 3.5 Å resolution using single-particle reconstruction methods, which share identical features with maps obtained by CTEM of a previously acquired same-sized TMV data set. These data show that STEM imaging in general, and in particular the iDPC-STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules.

PubMed: 36064775
DOI: 10.1038/s41592-022-01586-0

実験手法

ELECTRON MICROSCOPY (6.3 Å)