7Q1Z

Structure of formaldehyde cross-linked SARS-CoV-2 S glycoprotein

7Q1Z の概要

• エントリーDOI: 10.2210/pdb7q1z/pdb
• EMDBエントリー: 13776
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: sars-cov-2, glycoprotein, immunization, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 440864.00

構造登録者

Sulbaran, G.,Effantin, G.,Schoehn, G.,Weissenhorn, W. (登録日: 2021-10-22, 公開日: 2022-03-09, 最終更新日: 2025-07-09)

主引用文献

Sulbaran, G.,Maisonnasse, P.,Amen, A.,Effantin, G.,Guilligay, D.,Dereuddre-Bosquet, N.,Burger, J.A.,Poniman, M.,Grobben, M.,Buisson, M.,Dergan Dylon, S.,Naninck, T.,Lemaitre, J.,Gros, W.,Gallouet, A.S.,Marlin, R.,Bouillier, C.,Contreras, V.,Relouzat, F.,Fenel, D.,Thepaut, M.,Bally, I.,Thielens, N.,Fieschi, F.,Schoehn, G.,van der Werf, S.,van Gils, M.J.,Sanders, R.W.,Poignard, P.,Le Grand, R.,Weissenhorn, W.
Immunization with synthetic SARS-CoV-2 S glycoprotein virus-like particles protects macaques from infection.
Cell Rep Med, 3:100528-100528, 2022

PubMed Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused an ongoing global health crisis. Here, we present as a vaccine candidate synthetic SARS-CoV-2 spike (S) glycoprotein-coated lipid vesicles that resemble virus-like particles. Soluble S glycoprotein trimer stabilization by formaldehyde cross-linking introduces two major inter-protomer cross-links that keep all receptor-binding domains in the "down" conformation. Immunization of cynomolgus macaques with S coated onto lipid vesicles (S-LVs) induces high antibody titers with potent neutralizing activity against the vaccine strain, Alpha, Beta, and Gamma variants as well as T helper (Th)1 CD4-biased T cell responses. Although anti-receptor-binding domain (RBD)-specific antibody responses are initially predominant, the third immunization boosts significant non-RBD antibody titers. Challenging vaccinated animals with SARS-CoV-2 shows a complete protection through sterilizing immunity, which correlates with the presence of nasopharyngeal anti-S immunoglobulin G (IgG) and IgA titers. Thus, the S-LV approach is an efficient and safe vaccine candidate based on a proven classical approach for further development and clinical testing.

PubMed: 35233549
DOI: 10.1016/j.xcrm.2022.100528

実験手法

ELECTRON MICROSCOPY (3.4 Å)