7Q1S

A de novo designed hetero-dimeric antiparallel coiled coil apCC-Di-AB_var

7Q1S の概要

• エントリーDOI: 10.2210/pdb7q1s/pdb
• 分子名称: apCC-Di-B_var, apCC-Di-A_var, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: antiparallel, hetero-dimeric, coiled-coil, de novo, protein design, associating peptides, de novo protein
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 48174.97

構造登録者

Shanmugaratnam, S.,Rhys, G.G.,Dawson, W.M.,Woolfson, D.N.,Hocker, B. (登録日: 2021-10-20, 公開日: 2022-07-20, 最終更新日: 2024-10-23)

主引用文献

Rhys, G.G.,Cross, J.A.,Dawson, W.M.,Thompson, H.F.,Shanmugaratnam, S.,Savery, N.J.,Dodding, M.P.,Hocker, B.,Woolfson, D.N.
De novo designed peptides for cellular delivery and subcellular localisation.
Nat.Chem.Biol., 18:999-1004, 2022

PubMed Abstract: Increasingly, it is possible to design peptide and protein assemblies de novo from first principles or computationally. This approach provides new routes to functional synthetic polypeptides, including designs to target and bind proteins of interest. Much of this work has been developed in vitro. Therefore, a challenge is to deliver de novo polypeptides efficiently to sites of action within cells. Here we describe the design, characterisation, intracellular delivery, and subcellular localisation of a de novo synthetic peptide system. This system comprises a dual-function basic peptide, programmed both for cell penetration and target binding, and a complementary acidic peptide that can be fused to proteins of interest and introduced into cells using synthetic DNA. The designs are characterised in vitro using biophysical methods and X-ray crystallography. The utility of the system for delivery into mammalian cells and subcellular targeting is demonstrated by marking organelles and actively engaging functional protein complexes.

PubMed: 35836017
DOI: 10.1038/s41589-022-01076-6

実験手法

X-RAY DIFFRACTION (1.99 Å)