7Q0R

Structure of the Candida albicans 80S ribosome in complex with blasticidin s

This is a non-PDB format compatible entry.

Summary for 7Q0R

• Entry DOI: 10.2210/pdb7q0r/pdb
• Related: 7PZY 7Q08 7Q0F 7Q0P
• EMDB information: 13750
• Descriptor: 25S ribosomal RNA, Ribosomal 60S subunit protein L7A, 60S ribosomal protein L8, ... (83 entities in total)
• Functional Keywords: candida albicans, ribosome, blasticidin s, peptidyl transferase center
• Biological source: Candida albicans SC5314; More
• Total number of polymer chains: 80
• Total formula weight: 3145018.16

Authors

Kolosova, O.,Zgadzay, Y.,Stetsenko, A.,Jenner, L.,Guskov, A.,Yusupova, G.,Yusupov, M. (deposition date: 2021-10-16, release date: 2022-05-25, Last modification date: 2022-06-08)

Primary citation

Zgadzay, Y.,Kolosova, O.,Stetsenko, A.,Wu, C.,Bruchlen, D.,Usachev, K.,Validov, S.,Jenner, L.,Rogachev, A.,Yusupova, G.,Sachs, M.S.,Guskov, A.,Yusupov, M.
E-site drug specificity of the human pathogen Candida albicans ribosome.
Sci Adv, 8:eabn1062-eabn1062, 2022

PubMed Abstract: is a widespread commensal fungus with substantial pathogenic potential and steadily increasing resistance to current antifungal drugs. It is known to be resistant to cycloheximide (CHX) that binds to the E-transfer RNA binding site of the ribosome. Because of lack of structural information, it is neither possible to understand the nature of the resistance nor to develop novel inhibitors. To overcome this issue, we determined the structure of the vacant 80 ribosome at 2.3 angstroms and its complexes with bound inhibitors at resolutions better than 2.9 angstroms using cryo-electron microscopy. Our structures reveal how a change in a conserved amino acid in ribosomal protein eL42 explains CHX resistance in and forms a basis for further antifungal drug development.

PubMed: 35613268
DOI: 10.1126/sciadv.abn1062

Experimental method

ELECTRON MICROSCOPY (2.67 Å)