7Q0R

Structure of the Candida albicans 80S ribosome in complex with blasticidin s

これはPDB形式変換不可エントリーです。

7Q0R の概要

• エントリーDOI: 10.2210/pdb7q0r/pdb
• 関連するPDBエントリー: 7PZY 7Q08 7Q0F 7Q0P
• EMDBエントリー: 13750
• 分子名称: 25S ribosomal RNA, Ribosomal 60S subunit protein L7A, 60S ribosomal protein L8, ... (83 entities in total)
• 機能のキーワード: candida albicans, ribosome, blasticidin s, peptidyl transferase center
• 由来する生物種: Candida albicans SC5314; 詳細
• タンパク質・核酸の鎖数: 80
• 化学式量合計: 3145018.16

構造登録者

Kolosova, O.,Zgadzay, Y.,Stetsenko, A.,Jenner, L.,Guskov, A.,Yusupova, G.,Yusupov, M. (登録日: 2021-10-16, 公開日: 2022-05-25, 最終更新日: 2022-06-08)

主引用文献

Zgadzay, Y.,Kolosova, O.,Stetsenko, A.,Wu, C.,Bruchlen, D.,Usachev, K.,Validov, S.,Jenner, L.,Rogachev, A.,Yusupova, G.,Sachs, M.S.,Guskov, A.,Yusupov, M.
E-site drug specificity of the human pathogen Candida albicans ribosome.
Sci Adv, 8:eabn1062-eabn1062, 2022

PubMed Abstract: is a widespread commensal fungus with substantial pathogenic potential and steadily increasing resistance to current antifungal drugs. It is known to be resistant to cycloheximide (CHX) that binds to the E-transfer RNA binding site of the ribosome. Because of lack of structural information, it is neither possible to understand the nature of the resistance nor to develop novel inhibitors. To overcome this issue, we determined the structure of the vacant 80 ribosome at 2.3 angstroms and its complexes with bound inhibitors at resolutions better than 2.9 angstroms using cryo-electron microscopy. Our structures reveal how a change in a conserved amino acid in ribosomal protein eL42 explains CHX resistance in and forms a basis for further antifungal drug development.

PubMed: 35613268
DOI: 10.1126/sciadv.abn1062

実験手法

ELECTRON MICROSCOPY (2.67 Å)