7PZ2

Structure of the mechanosensor domain of Wsc1 from Saccharomyces cerevisiae

7PZ2 の概要

• エントリーDOI: 10.2210/pdb7pz2/pdb
• 分子名称: HN1_G0037740.mRNA.1.CDS.1, CHLORIDE ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: mechanosensor domain, cell wall, yeast, signal transduction, cell adhesion
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25946.18

構造登録者

Schoeppner, P.,Mosch, H.U.,Essen, L.O. (登録日: 2021-10-11, 公開日: 2022-11-02, 最終更新日: 2024-11-20)

主引用文献

Schoppner, P.,Lutz, A.P.,Lutterbach, B.J.,Bruckner, S.,Essen, L.O.,Mosch, H.U.
Structure of the Yeast Cell Wall Integrity Sensor Wsc1 Reveals an Essential Role of Surface-Exposed Aromatic Clusters.
J Fungi, 8:-, 2022

PubMed Abstract: In the yeast and other ascomycetes, the maintenance of cell wall integrity is governed by a family of plasma-membrane spanning sensors that include the Wsc-type proteins. These cell wall proteins apparently sense stress-induced mechanical forces at the cell surface and target the cell wall integrity (CWI) signaling pathway, but the structural base for their sensor function is yet unknown. Here, we solved a high-resolution crystal structure of the extracellular cysteine-rich domain (CRD) of yeast Wsc1, which shows the characteristic PAN/Apple domain fold with two of the four Wsc1 disulfide bridges being conserved in other PAN domain cores. Given the general function of PAN domains in mediating protein-protein and protein-carbohydrate interactions, this finding underpins the importance of Wsc domains in conferring sensing and localization functions. Our Wsc1 CRD structure reveals an unusually high number of surface-exposed aromatic residues that are conserved in other fungal CRDs, and can be arranged into three solvent-exposed clusters. Mutational analysis demonstrates that two of the aromatic clusters are required for conferring Wsc1-dependent resistance to the glucan synthase inhibitor caspofungin, and the chitin-binding agents Congo red and Calcofluor white. These findings suggest an essential role of surface-exposed aromatic clusters in fungal Wsc-type sensors that might include an involvement in stress-induced sensor-clustering required to elicit appropriate cellular responses via the downstream CWI pathway.

PubMed: 35448610
DOI: 10.3390/jof8040379

実験手法

X-RAY DIFFRACTION (1.58 Å)