7PTV

Structure of the Mimivirus genomic fibre asymmetric unit

7PTV の概要

• エントリーDOI: 10.2210/pdb7ptv/pdb
• 関連するPDBエントリー: 4z24
• EMDBエントリー: 13641
• 分子名称: Putative glucose-methanol-choline oxidoreductase protein, FLAVIN-ADENINE DINUCLEOTIDE (2 entities in total)
• 機能のキーワード: mimivirus, genomic fibre, cytoplasmic infectious cycle, 1.2 mb dsdna, virus, viral protein
• 由来する生物種: Acanthamoeba polyphaga mimivirus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 154433.21

構造登録者

Villalta, A.,Schmitt, A.,Estrozi, L.F.,Quemin, E.R.J.,Alempic, J.M.,Lartigue, A.,Prazak, V.,Belmudes, L.,Vasishtan, D.,Colmant, A.M.G.,Honore, F.A.,Coute, Y.,Grunewald, K.,Abergel, C. (登録日: 2021-09-27, 公開日: 2022-08-10, 最終更新日: 2024-07-17)

主引用文献

Villalta, A.,Schmitt, A.,Estrozi, L.F.,Quemin, E.R.J.,Alempic, J.M.,Lartigue, A.,Prazak, V.,Belmudes, L.,Vasishtan, D.,Colmant, A.M.G.,Honore, F.A.,Coute, Y.,Grunewald, K.,Abergel, C.
The giant mimivirus 1.2 Mb genome is elegantly organized into a 30 nm diameter helical protein shield.
Elife, 11:-, 2022

PubMed Abstract: Mimivirus is the prototype of the family of giant dsDNA viruses. Little is known about the organization of the 1.2 Mb genome inside the membrane-limited nucleoid filling the ~0.5 µm icosahedral capsids. Cryo-electron microscopy, cryo-electron tomography, and proteomics revealed that it is encased into a ~30-nm diameter helical protein shell surprisingly composed of two GMC-type oxidoreductases, which also form the glycosylated fibrils decorating the capsid. The genome is arranged in 5- or 6-start left-handed super-helices, with each DNA-strand lining the central channel. This luminal channel of the nucleoprotein fiber is wide enough to accommodate oxidative stress proteins and RNA polymerase subunits identified by proteomics. Such elegant supramolecular organization would represent a remarkable evolutionary strategy for packaging and protecting the genome, in a state ready for immediate transcription upon unwinding in the host cytoplasm. The parsimonious use of the same protein in two unrelated substructures of the virion is unexpected for a giant virus with thousand genes at its disposal.

PubMed: 35900198
DOI: 10.7554/eLife.77607

実験手法

ELECTRON MICROSCOPY (3.3 Å)