7PTS

RNA origami 5-helix tile

7PTS の概要

• エントリーDOI: 10.2210/pdb7pts/pdb
• EMDBエントリー: 13636
• 分子名称: 5HT-B (1 entity in total)
• 機能のキーワード: rna, origami, nanostructure
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 179904.22

構造登録者

McRae, E.K.S.,Andersen, E.S. (登録日: 2021-09-27, 公開日: 2022-10-05, 最終更新日: 2024-07-17)

主引用文献

McRae, E.K.S.,Rasmussen, H.O.,Liu, J.,Boggild, A.,Nguyen, M.T.A.,Sampedro Vallina, N.,Boesen, T.,Pedersen, J.S.,Ren, G.,Geary, C.,Andersen, E.S.
Structure, folding and flexibility of co-transcriptional RNA origami.
Nat Nanotechnol, 18:808-817, 2023

PubMed Abstract: RNA origami is a method for designing RNA nanostructures that can self-assemble through co-transcriptional folding with applications in nanomedicine and synthetic biology. However, to advance the method further, an improved understanding of RNA structural properties and folding principles is required. Here we use cryogenic electron microscopy to study RNA origami sheets and bundles at sub-nanometre resolution revealing structural parameters of kissing-loop and crossover motifs, which are used to improve designs. In RNA bundle designs, we discover a kinetic folding trap that forms during folding and is only released after 10 h. Exploration of the conformational landscape of several RNA designs reveal the flexibility of helices and structural motifs. Finally, sheets and bundles are combined to construct a multidomain satellite shape, which is characterized by individual-particle cryo-electron tomography to reveal the domain flexibility. Together, the study provides a structural basis for future improvements to the design cycle of genetically encoded RNA nanodevices.

PubMed: 36849548
DOI: 10.1038/s41565-023-01321-6

実験手法

ELECTRON MICROSCOPY (5.71 Å)