7PSL

S. cerevisiae Atm1 in MSP1D1 nanodiscs in nucleotide-free state

7PSL の概要

• エントリーDOI: 10.2210/pdb7psl/pdb
• EMDBエントリー: 13613
• 分子名称: Iron-sulfur clusters transporter ATM1, mitochondrial, (1R)-2-{[(R)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(DODECANOYLOXY)METHYL]ETHYL (9Z)-OCTADEC-9-ENOATE, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: abc transporter, transport protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 138299.66

構造登録者

Ellinghaus, T.L.,Kuehlbrandt, W. (登録日: 2021-09-23, 公開日: 2021-12-29, 最終更新日: 2024-07-17)

主引用文献

Ellinghaus, T.L.,Marcellino, T.,Srinivasan, V.,Lill, R.,Kuhlbrandt, W.
Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle.
Sci Adv, 7:eabk2392-eabk2392, 2021

PubMed Abstract: The mitochondrial inner membrane ABC transporter Atm1 exports an unknown substrate to the cytosol for iron-sulfur protein biogenesis, cellular iron regulation, and tRNA thio-modification. Mutations in the human relative ABCB7 cause the iron storage disease XLSA/A. We determined 3D structures of two complementary states of Atm1 in lipid nanodiscs by electron cryo-microscopy at 2.9- to 3.4-Å resolution. The inward-open structure resembled the known crystal structure of nucleotide-free apo-Atm1 closely. The occluded conformation with bound AMP-PNP-Mg showed a tight association of the two nucleotide-binding domains, a rearrangement of the C-terminal helices, and closure of the putative substrate-binding cavity in the homodimeric transporter. We identified a hydrophobic patch on the C-terminal helices of yeast Atm1, which is unique among type IV ABC transporters of known structure. Truncation mutants of yeast Atm1 suggest that the C-terminal helices stabilize the dimer, yet are not necessary for closure of the nucleotide-binding domains.

PubMed: 34936443
DOI: 10.1126/sciadv.abk2392

実験手法

ELECTRON MICROSCOPY (3.3 Å)