7PS3

Crystal structure of antibody Beta-32 Fab

7PS3 の概要

• エントリーDOI: 10.2210/pdb7ps3/pdb
• 関連するPDBエントリー: 7PS2
• 分子名称: Beta-32 heavy chain, Beta-32 light chain, D-MALATE, ... (6 entities in total)
• 機能のキーワード: sars-cov-2 b.1.1.7 (alpha) variant, b.1.351 (beta) variant, p.1 (gamma) variant, b.1.617.2 (delta) variant, antibody, receptor-binding-domain, spike, neutralisation, viral protein/immune system, viral protein, immune system, viral protein-immune system complex
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48605.17

構造登録者

Zhou, D.,Ren, J.,Stuart, D.I. (登録日: 2021-09-22, 公開日: 2021-12-15, 最終更新日: 2024-10-16)

主引用文献

Liu, C.,Zhou, D.,Nutalai, R.,Duyvesteyn, H.M.E.,Tuekprakhon, A.,Ginn, H.M.,Dejnirattisai, W.,Supasa, P.,Mentzer, A.J.,Wang, B.,Case, J.B.,Zhao, Y.,Skelly, D.T.,Chen, R.E.,Johnson, S.A.,Ritter, T.G.,Mason, C.,Malik, T.,Temperton, N.,Paterson, N.G.,Williams, M.A.,Hall, D.R.,Clare, D.K.,Howe, A.,Goulder, P.J.R.,Fry, E.E.,Diamond, M.S.,Mongkolsapaya, J.,Ren, J.,Stuart, D.I.,Screaton, G.R.
The antibody response to SARS-CoV-2 Beta underscores the antigenic distance to other variants.
Cell Host Microbe, 30:53-, 2022

PubMed Abstract: Alpha-B.1.1.7, Beta-B.1.351, Gamma-P.1, and Delta-B.1.617.2 variants of SARS-CoV-2 express multiple mutations in the spike protein (S). These may alter the antigenic structure of S, causing escape from natural or vaccine-induced immunity. Beta is particularly difficult to neutralize using serum induced by early pandemic SARS-CoV-2 strains and is most antigenically separated from Delta. To understand this, we generated 674 mAbs from Beta-infected individuals and performed a detailed structure-function analysis of the 27 most potent mAbs: one binding the spike N-terminal domain (NTD), the rest the receptor-binding domain (RBD). Two of these RBD-binding mAbs recognize a neutralizing epitope conserved between SARS-CoV-1 and -2, while 18 target mutated residues in Beta: K417N, E484K, and N501Y. There is a major response to N501Y, including a public IgVH4-39 sequence, with E484K and K417N also targeted. Recognition of these key residues underscores why serum from Beta cases poorly neutralizes early pandemic and Delta viruses.

PubMed: 34921776
DOI: 10.1016/j.chom.2021.11.013

実験手法

X-RAY DIFFRACTION (1.7 Å)