7PR2

Cocrystal of cytochrome c and sulfonato-thiacalix[4]arene

7PR2 の概要

• エントリーDOI: 10.2210/pdb7pr2/pdb
• 分子名称: Cytochrome c iso-1, HEME C, sulfonato-thiacalix[4]arene, ... (4 entities in total)
• 機能のキーワード: calixarene, molecular glue, synthetic receptor, alpha helix, thiacalixarene, electron transport
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27771.23

構造登録者

Flood, R.J.,Ramberg, K.,Guagnini, F.,Crowley, P.B. (登録日: 2021-09-20, 公開日: 2022-03-02, 最終更新日: 2024-10-16)

主引用文献

Flood, R.J.,Ramberg, K.O.,Mengel, D.B.,Guagnini, F.,Crowley, P.B.
Protein Frameworks with Thiacalixarene and Zinc.
Cryst.Growth Des., 22:3271-3276, 2022

PubMed Abstract: Controlled protein assembly provides a means to generate biomaterials. Synthetic macrocycles such as the water-soluble sulfonato-calix[n]arenes are useful mediators of protein assembly. Sulfonato-thiacalix[4]arene ( ), with its metal-binding capacity, affords the potential for simultaneous macrocycle- and metal-mediated protein assembly. Here, we describe the -/Zn-directed assembly of two proteins: cationic α-helical cytochrome (cyt ) and neutral β-propeller lectin (RSL). Two co-crystal forms were obtained with cyt , each involving multinuclear zinc sites supported by the cone conformation of . The /Zn cluster acted as an assembly node via both lysine encapsulation and metal-mediated protein-protein contacts. In the case of RSL, adopted the 1,2-alternate conformation and supported a dinuclear zinc site with concomitant encapsulation and metal-binding of two histidine side chains. These results, together with the knowledge of thiacalixarene/metal nanoclusters, suggest promising applications for thiacalixarenes in biomaterials and MOF fabrication.

PubMed: 35529063
DOI: 10.1021/acs.cgd.2c00108

実験手法

X-RAY DIFFRACTION (1.73 Å)