7PQ9

Crystal structure of Bacillus clausii pdxR at 2.8 Angstroms resolution

これはPDB形式変換不可エントリーです。

7PQ9 の概要

• エントリーDOI: 10.2210/pdb7pq9/pdb
• 分子名称: PLP-dependent aminotransferase family protein, CALCIUM ION, CHLORIDE ION, ... (8 entities in total)
• 機能のキーワード: bacillus clausii; mocr; pdxr; pyridoxal 5'-phosphate biosynthesis; transcriptional regulation; apo, dna binding protein
• 由来する生物種: Alkalihalobacillus clausii
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 609690.04

構造登録者

Vivoli Vega, M.,Isupov, M.N.,Harmer, N. (登録日: 2021-09-16, 公開日: 2022-09-28, 最終更新日: 2024-01-31)

主引用文献

Freda, I.,Exertier, C.,Barile, A.,Chaves-Sanjuan, A.,Vega, M.V.,Isupov, M.N.,Harmer, N.J.,Gugole, E.,Swuec, P.,Bolognesi, M.,Scipioni, A.,Savino, C.,Di Salvo, M.L.,Contestabile, R.,Vallone, B.,Tramonti, A.,Montemiglio, L.C.
Structural insights into the DNA recognition mechanism by the bacterial transcription factor PdxR.
Nucleic Acids Res., 51:8237-8254, 2023

PubMed Abstract: Specificity in protein-DNA recognition arises from the synergy of several factors that stem from the structural and chemical signatures encoded within the targeted DNA molecule. Here, we deciphered the nature of the interactions driving DNA recognition and binding by the bacterial transcription factor PdxR, a member of the MocR family responsible for the regulation of pyridoxal 5'-phosphate (PLP) biosynthesis. Single particle cryo-EM performed on the PLP-PdxR bound to its target DNA enabled the isolation of three conformers of the complex, which may be considered as snapshots of the binding process. Moreover, the resolution of an apo-PdxR crystallographic structure provided a detailed description of the transition of the effector domain to the holo-PdxR form triggered by the binding of the PLP effector molecule. Binding analyses of mutated DNA sequences using both wild type and PdxR variants revealed a central role of electrostatic interactions and of the intrinsic asymmetric bending of the DNA in allosterically guiding the holo-PdxR-DNA recognition process, from the first encounter through the fully bound state. Our results detail the structure and dynamics of the PdxR-DNA complex, clarifying the mechanism governing the DNA-binding mode of the holo-PdxR and the regulation features of the MocR family of transcription factors.

PubMed: 37378428
DOI: 10.1093/nar/gkad552

実験手法

X-RAY DIFFRACTION (2.8 Å)