7POC

An Irreversible, Promiscuous and Highly Thermostable Claisen-Condensation Biocatalyst Drives the Synthesis of Substituted Pyrroles

7POC の概要

• エントリーDOI: 10.2210/pdb7poc/pdb
• 分子名称: 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase, PYRIDOXAL-5'-PHOSPHATE (3 entities in total)
• 機能のキーワード: biocatalyst, cascade, chemo-enzymatic, thermophilic, promiscuous, alpha-oxoamine synthase, pyrrole, synthase
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 187329.15

構造登録者

Basle, A.,Ashley, B.,Campopiano, D.,Marles-Wright, J. (登録日: 2021-09-08, 公開日: 2022-09-21, 最終更新日: 2024-02-28)

主引用文献

Ashley, B.,Basle, A.,Sajjad, M.,El Ashram, A.,Kelis, P.,Marles-Wright, J.,Campopiano, D.J.
Versatile Chemo-Biocatalytic Cascade Driven by a Thermophilic and Irreversible C-C Bond-Forming alpha-Oxoamine Synthase.
Acs Sustain Chem Eng, 11:7997-8002, 2023

PubMed Abstract: We report a chemo-biocatalytic cascade for the synthesis of substituted pyrroles, driven by the action of an irreversible, thermostable, pyridoxal 5'-phosphate (PLP)-dependent, C-C bond-forming biocatalyst (AOS). The AOS catalyzes the Claisen-like condensation between various amino acids and acyl-CoA substrates to generate a range of α-aminoketones. These products are reacted with β-keto esters in an irreversible Knorr pyrrole reaction. The determination of the 1.6 Å resolution crystal structure of the PLP-bound form of AOS lays the foundation for future engineering and directed evolution. This report establishes the AOS family as useful and versatile C-C bond-forming biocatalysts.

PubMed: 37266354
DOI: 10.1021/acssuschemeng.3c00243

実験手法

X-RAY DIFFRACTION (2.6 Å)