7PM3

Cryo-EM structure of young JASP-stabilized F-actin (central 3er)

7PM3 の概要

• エントリーDOI: 10.2210/pdb7pm3/pdb
• 関連するPDBエントリー: 7PLY 7PLZ
• EMDBエントリー: 13511
• 分子名称: Actin, alpha skeletal muscle, ADENOSINE-5'-DIPHOSPHATE, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: structural protein, myosin, cytoskeleton, f-actin, jasplakinolide
• 由来する生物種: Oryctolagus cuniculus (Rabbit)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 130098.36

構造登録者

Pospich, S.,Sweeney, H.L.,Houdusse, A.,Raunser, S. (登録日: 2021-09-01, 公開日: 2021-12-22)

主引用文献

Pospich, S.,Sweeney, H.L.,Houdusse, A.,Raunser, S.
High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.
Elife, 10:-, 2021

PubMed Abstract: The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding are only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin.

PubMed: 34812732
DOI: 10.7554/eLife.73724

実験手法

ELECTRON MICROSCOPY (3.1 Å)