7PKZ

Vault structure in committed conformation

これはPDB形式変換不可エントリーです。

7PKZ の概要

• エントリーDOI: 10.2210/pdb7pkz/pdb
• EMDBエントリー: 13483
• 分子名称: Major vault protein (1 entity in total)
• 機能のキーワード: transport, structural protein
• 由来する生物種: Rattus norvegicus (Rat)
• タンパク質・核酸の鎖数: 78
• 化学式量合計: 7481920.84

構造登録者

Guerra, P.,Gonzalez-Alamos, M.,Llauro, A.,Casanas, A.,Querol-Audi, J.,de Pablo, P.,Verdaguer, N. (登録日: 2021-08-27, 公開日: 2022-03-16, 最終更新日: 2024-07-17)

主引用文献

Guerra, P.,Gonzalez-Alamos, M.,Llauro, A.,Casanas, A.,Querol-Audi, J.,de Pablo, P.J.,Verdaguer, N.
Symmetry disruption commits vault particles to disassembly.
Sci Adv, 8:eabj7795-eabj7795, 2022

PubMed Abstract: Vaults are ubiquitous ribonucleoprotein particles involved in a diversity of cellular processes, with promising applications as nanodevices for delivery of multiple cargos. The vault shell is assembled by the symmetrical association of multiple copies of the major vault protein that, initially, generates half vaults. The pairwise, anti-parallel association of two half vaults produces whole vaults. Here, using a combination of vault recombinant reconstitution and structural techniques, we characterized the molecular determinants for the vault opening process. This process commences with a relaxation of the vault waist, causing the expansion of the inner cavity. Then, local disengagement of amino-terminal domains at the vault midsection seeds a conformational change that leads to the aperture, facilitating access to the inner cavity where cargo is hosted. These results inform a hitherto uncharacterized step of the vault cycle and will aid current engineering efforts leveraging vault for tailored cargo delivery.

PubMed: 35138889
DOI: 10.1126/sciadv.abj7795

実験手法

ELECTRON MICROSCOPY (9.8 Å)