7PKS

Structural basis of Integrator-mediated transcription regulation

これはPDB形式変換不可エントリーです。

7PKS の概要

• エントリーDOI: 10.2210/pdb7pks/pdb
• EMDBエントリー: 13479
• 分子名称: DNA-directed RNA polymerase subunit, DNA-directed RNA polymerases I, II, and III subunit RPABC5, RNA_pol_L_2 domain-containing protein, ... (36 entities in total)
• 機能のキーワード: promoter proximal pausing, attenuation, snrna termination, integrator complex, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 33
• 化学式量合計: 2033399.18

構造登録者

Fianu, I.,Chen, Y.,Dienemann, C.,Cramer, P. (登録日: 2021-08-26, 公開日: 2021-12-08, 最終更新日: 2024-10-09)

主引用文献

Fianu, I.,Chen, Y.,Dienemann, C.,Dybkov, O.,Linden, A.,Urlaub, H.,Cramer, P.
Structural basis of Integrator-mediated transcription regulation.
Science, 374:883-887, 2021

PubMed Abstract: Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves the nascent RNA, and terminates transcription. We report the structure of the pretermination complex containing the human Integrator-PP2A complex bound to paused Pol II. Integrator binds Pol II and the pausing factors DSIF and NELF to exclude binding of the elongation factors SPT6 and PAF1 complex. Integrator also binds the C-terminal domain of Pol II and positions PP2A to counteract Pol II phosphorylation and elongation. The Integrator endonuclease docks to the RNA exit site and opens to cleave nascent RNA about 20 nucleotides from the Pol II active site. Integrator does not bind the DNA clamps formed by Pol II and DSIF, enabling release of DNA and transcription termination.

PubMed: 34762484
DOI: 10.1126/science.abk0154

実験手法

ELECTRON MICROSCOPY (3.6 Å)