7PJD

The X-ray structure of juvenile hormone diol kinase from the silk worm Bombyx mori.

7PJD の概要

• エントリーDOI: 10.2210/pdb7pjd/pdb
• 分子名称: Juvenile hormone diol kinase, CALCIUM ION (3 entities in total)
• 機能のキーワード: ef-hand, calcium binding, kinase, juvenile hormone metabolism. metal binding protein, metal binding protein
• 由来する生物種: Bombyx mori (domestic silkworm)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 125792.50

構造登録者

Guo, J.,Cooper, J.,Keegan, R.M. (登録日: 2021-08-23, 公開日: 2021-12-22, 最終更新日: 2024-10-16)

主引用文献

Guo, J.,Keegan, R.M.,Rigden, D.J.,Erskine, P.T.,Wood, S.P.,Li, S.,Cooper, J.B.
The X-ray structure of juvenile hormone diol kinase from the silkworm Bombyx mori.
Acta Crystallogr.,Sect.F, 77:465-472, 2021

PubMed Abstract: Insect juvenile hormones (JHs) are a family of sesquiterpenoid molecules that are secreted into the haemolymph. JHs have multiple roles in insect development, metamorphosis and sexual maturation. A number of pesticides work by chemically mimicking JHs, thus preventing insects from developing and reproducing normally. The haemolymph levels of JH are governed by the rates of its biosynthesis and degradation. One enzyme involved in JH catabolism is JH diol kinase (JHDK), which uses ATP (or GTP) to phosphorylate JH diol to JH diol phosphate, which can be excreted. The X-ray structure of JHDK from the silkworm Bombyx mori has been determined at a resolution of 2.0 Å with an R factor of 19.0% and an R of 24.8%. The structure possesses three EF-hand motifs which are occupied by calcium ions. This is in contrast to the recently reported structure of the JHDK-like-2 protein from B. mori (PDB entry 6kth), which possessed only one calcium ion. Since JHDK is known to be inhibited by calcium ions, it is likely that our structure represents the calcium-inhibited form of the enzyme. The electrostatic surface of the protein suggests a binding site for the triphosphate of ATP close to the N-terminal end of the molecule in a cavity between the N- and C-terminal domains. Superposition with a number of calcium-activated photoproteins suggests that there may be parallels between the binding of JH diol to JHDK and the binding of luciferin to aequorin.

PubMed: 34866602
DOI: 10.1107/S2053230X21012012

実験手法

X-RAY DIFFRACTION (1.99 Å)