7PFO

Core human replisome

これはPDB形式変換不可エントリーです。

7PFO の概要

• エントリーDOI: 10.2210/pdb7pfo/pdb
• EMDBエントリー: 13375 13376 13377 13384 13457
• 分子名称: DNA replication licensing factor MCM2, DNA replication complex GINS protein PSF1, DNA replication complex GINS protein PSF2, ... (23 entities in total)
• 機能のキーワード: dna replication, helicase, cmg, timeless, tipin, claspin, and-1, epsilon, polymerase, replisome, replication
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 1797086.04

構造登録者

Jones, M.J.,Yeeles, J.T.P. (登録日: 2021-08-11, 公開日: 2021-11-10, 最終更新日: 2025-07-09)

主引用文献

Jones, M.L.,Baris, Y.,Taylor, M.R.G.,Yeeles, J.T.P.
Structure of a human replisome shows the organisation and interactions of a DNA replication machine.
Embo J., 40:e108819-e108819, 2021

PubMed Abstract: The human replisome is an elaborate arrangement of molecular machines responsible for accurate chromosome replication. At its heart is the CDC45-MCM-GINS (CMG) helicase, which, in addition to unwinding the parental DNA duplex, arranges many proteins including the leading-strand polymerase Pol ε, together with TIMELESS-TIPIN, CLASPIN and AND-1 that have key and varied roles in maintaining smooth replisome progression. How these proteins are coordinated in the human replisome is poorly understood. We have determined a 3.2 Å cryo-EM structure of a human replisome comprising CMG, Pol ε, TIMELESS-TIPIN, CLASPIN and AND-1 bound to replication fork DNA. The structure permits a detailed understanding of how AND-1, TIMELESS-TIPIN and Pol ε engage CMG, reveals how CLASPIN binds to multiple replisome components and identifies the position of the Pol ε catalytic domain. Furthermore, the intricate network of contacts contributed by MCM subunits and TIMELESS-TIPIN with replication fork DNA suggests a mechanism for strand separation.

PubMed: 34694004
DOI: 10.15252/embj.2021108819

実験手法

ELECTRON MICROSCOPY (3.2 Å)