7PEO

Structure of the Caulobacter crescentus S-layer protein RsaA N-terminal domain bound to LPS and soaked with Holmium

Summary for 7PEO

• Entry DOI: 10.2210/pdb7peo/pdb
• EMDB information: 10389 13355
• Descriptor: S-layer protein, 4-acetamido-4,6-dideoxy-alpha-D-mannopyranose-(1-3)-4-acetamido-4,6-dideoxy-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-3)-4-acetamido-4,6-dideoxy-alpha-D-mannopyranose-(1-3)-4-acetamido-4,6-dideoxy-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-3)-4-acetamido-4,6-dideoxy-alpha-D-mannopyranose-(1-3)-4-acetamido-4,6-dideoxy-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-3)-4-acetamido-4,6-dideoxy-alpha-D-mannopyranose-(1-3)-4-acetamido-4,6-dideoxy-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: s-layer protein rsaa bound to lps and holmium, structural protein
• Biological source: Caulobacter vibrioides
• Total number of polymer chains: 1
• Total formula weight: 28229.56

Authors

von Kugelgen, A.,Bharat, T.A.M. (deposition date: 2021-08-11, release date: 2021-12-01, Last modification date: 2024-07-17)

Primary citation

Herdman, M.,von Kugelgen, A.,Kureisaite-Ciziene, D.,Duman, R.,El Omari, K.,Garman, E.F.,Kjaer, A.,Kolokouris, D.,Lowe, J.,Wagner, A.,Stansfeld, P.J.,Bharat, T.A.M.
High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.
Structure, 30:215-, 2022

PubMed Abstract: Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in the formation of the Caulobacter crescentus S-layer using high-resolution structural and cell biology techniques, as well as molecular simulations. Utilizing optical microscopy of fluorescently tagged S-layers, we show that calcium ions facilitate S-layer lattice formation and cell-surface binding. We report all-atom molecular dynamics simulations of the S-layer lattice, revealing the importance of bound metal ions. Finally, using electron cryomicroscopy and long-wavelength X-ray diffraction experiments, we mapped the positions of metal ions in the S-layer at near-atomic resolution, supporting our insights from the cellular and simulations data. Our findings contribute to the understanding of how C. crescentus cells form a regularly arranged S-layer on their surface, with implications on fundamental S-layer biology and the synthetic biology of self-assembling biomaterials.

PubMed: 34800371
DOI: 10.1016/j.str.2021.10.012

Experimental method

ELECTRON MICROSCOPY (4.37 Å)