7PC2
HIV-1 Env (BG505 SOSIP.664) in complex with the IgA bNAb 7-269 and the antibody 3BNC117.
Summary for 7PC2
| Entry DOI | 10.2210/pdb7pc2/pdb |
| EMDB information | 13316 |
| Descriptor | 7-269 IgA Fab heavy chain, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (15 entities in total) |
| Functional Keywords | hiv, antibody, bnab, env, viral protein |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 18 |
| Total formula weight | 546142.23 |
| Authors | Fernandez, I.,Bontems, F.,Pehau-Arnaudet, G.,Rey, F. (deposition date: 2021-08-03, release date: 2022-02-23, Last modification date: 2024-11-06) |
| Primary citation | Lorin, V.,Fernandez, I.,Masse-Ranson, G.,Bouvin-Pley, M.,Molinos-Albert, L.M.,Planchais, C.,Hieu, T.,Pehau-Arnaudet, G.,Hrebik, D.,Girelli-Zubani, G.,Fiquet, O.,Guivel-Benhassine, F.,Sanders, R.W.,Walker, B.D.,Schwartz, O.,Scheid, J.F.,Dimitrov, J.D.,Plevka, P.,Braibant, M.,Seaman, M.S.,Bontems, F.,Di Santo, J.P.,Rey, F.A.,Mouquet, H. Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller. J.Exp.Med., 219:-, 2022 Cited by PubMed Abstract: Decrypting the B cell ontogeny of HIV-1 broadly neutralizing antibodies (bNAbs) is paramount for vaccine design. Here, we characterized IgA and IgG bNAbs of three distinct B cell lineages in a viremic controller, two of which comprised only IgG+ or IgA+ blood memory B cells; the third combined both IgG and IgA clonal variants. 7-269 bNAb in the IgA-only lineage displayed the highest neutralizing capacity despite limited somatic mutation, and delayed viral rebound in humanized mice. bNAbs in all three lineages targeted the N332 glycan supersite. The 2.8-Å resolution cryo-EM structure of 7-269-BG505 SOSIP.664 complex showed a similar pose as 2G12, on an epitope mainly composed of sugar residues comprising the N332 and N295 glycans. Binding and cryo-EM structural analyses showed that antibodies from the two other lineages interact mostly with glycans N332 and N386. Hence, multiple B cell lineages of IgG and IgA bNAbs focused on a unique HIV-1 site of vulnerability can codevelop in HIV-1 viremic controllers. PubMed: 35230385DOI: 10.1084/jem.20212045 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.8 Å) |
Structure validation
Download full validation report
