7P98

Cyclohex-1-ene-1-carboxyl-CoA dehydrogenase in a substrate-free state

7P98 の概要

• エントリーDOI: 10.2210/pdb7p98/pdb
• 分子名称: Short-chain acyl-CoA dehydrogenase, FLAVIN-ADENINE DINUCLEOTIDE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: enzyme catalysis, acyl-coa dehydrogenase, oxidoreductase, flavin, fatty acid oxidation
• 由来する生物種: Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91018.04

構造登録者

Ermler, U.,Weidenweber, S.,Boll, M. (登録日: 2021-07-26, 公開日: 2022-07-13, 最終更新日: 2024-01-31)

主引用文献

Kung, J.W.,Meier, A.K.,Willistein, M.,Weidenweber, S.,Demmer, U.,Ermler, U.,Boll, M.
Structural Basis of Cyclic 1,3-Diene Forming Acyl-Coenzyme A Dehydrogenases.
Chembiochem, 22:3173-3177, 2021

PubMed Abstract: The biologically important, FAD-containing acyl-coenzyme A (CoA) dehydrogenases (ACAD) usually catalyze the anti-1,2-elimination of a proton and a hydride of aliphatic CoA thioesters. Here, we report on the structure and function of an ACAD from anaerobic bacteria catalyzing the unprecedented 1,4-elimination at C3 and C6 of cyclohex-1-ene-1-carboxyl-CoA (Ch1CoA) to cyclohex-1,5-diene-1-carboxyl-CoA (Ch1,5CoA) and at C3 and C4 of the latter to benzoyl-CoA. Based on high-resolution Ch1CoA dehydrogenase crystal structures, the unorthodox reactivity is explained by the presence of a catalytic aspartate base (D91) at C3, and by eliminating the catalytic glutamate base at C1. Moreover, C6 of Ch1CoA and C4 of Ch1,5CoA are positioned towards FAD-N5 to favor the biologically relevant C3,C6- over the C3,C4-dehydrogenation activity. The C1,C2-dehydrogenation activity was regained by structure-inspired amino acid exchanges. The results provide the structural rationale for the extended catalytic repertoire of ACADs and offer previously unknown biocatalytic options for the synthesis of cyclic 1,3-diene building blocks.

PubMed: 34555236
DOI: 10.1002/cbic.202100421

実験手法

X-RAY DIFFRACTION (2 Å)