7P93

Crystal Structure of leukotoxin LukE from Staphylococcus aureus in complex with a sulfated ACKR1 N-terminal peptide

7P93 の概要

• エントリーDOI: 10.2210/pdb7p93/pdb
• 分子名称: Leucotoxin LukEv, Atypical chemokine receptor 1 (3 entities in total)
• 機能のキーワード: leukotoxin, beta barrel pore forming toxin, cytolysis, hemolysis, toxin
• 由来する生物種: Staphylococcus aureus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 37697.64

構造登録者

Lambey, P.,Hoh, F.,Peysson, F.,Granier, S.,Leyrat, C. (登録日: 2021-07-23, 公開日: 2022-04-06, 最終更新日: 2024-11-06)

主引用文献

Lambey, P.,Otun, O.,Cong, X.,Hoh, F.,Brunel, L.,Verdie, P.,Grison, C.M.,Peysson, F.,Jeannot, S.,Durroux, T.,Bechara, C.,Granier, S.,Leyrat, C.
Structural insights into recognition of chemokine receptors by Staphylococcus aureus leukotoxins.
Elife, 11:-, 2022

PubMed Abstract: (SA) leukocidin ED (LukED) belongs to a family of bicomponent pore forming toxins that play important roles in SA immune evasion and nutrient acquisition. LukED targets specific G protein-coupled chemokine receptors to lyse human erythrocytes (red blood cells) and leukocytes (white blood cells). The first recognition step of receptors is critical for specific cell targeting and lysis. The structural and molecular bases for this mechanism are not well understood but could constitute essential information to guide antibiotic development. Here, we characterized the interaction of LukE with chemokine receptors ACKR1, CCR2, and CCR5 using a combination of structural, pharmacological, and computational approaches. First, crystal structures of LukE in complex with a small molecule mimicking sulfotyrosine side chain (p-cresyl sulfate) and with peptides containing sulfotyrosines issued from receptor sequences revealed the location of receptor sulfotyrosine binding sites in the toxins. Then, by combining previous and novel experimental data with protein docking, classical and accelerated weight histogram (AWH) molecular dynamics we propose models of the ACKR1-LukE and CCR5-LukE complexes. This work provides novel insights into chemokine receptor recognition by leukotoxins and suggests that the conserved sulfotyrosine binding pocket could be a target of choice for future drug development.

PubMed: 35311641
DOI: 10.7554/eLife.72555

実験手法

X-RAY DIFFRACTION (1.55 Å)