7P6Z

Mycoplasma pneumoniae 70S ribosome in untreated cells

これはPDB形式変換不可エントリーです。

7P6Z の概要

• エントリーDOI: 10.2210/pdb7p6z/pdb
• EMDBエントリー: 13234
• 分子名称: 23S ribosomal RNA, 50S ribosomal protein L21, 50S ribosomal protein L27, ... (57 entities in total)
• 機能のキーワード: in-cell cryo-electron tomography, sub-tomogram analysis, ribosome
• 由来する生物種: Mycoplasma pneumoniae M129; 詳細
• タンパク質・核酸の鎖数: 55
• 化学式量合計: 2250563.27

構造登録者

Xue, L.,Lenz, S.,Rappsilber, J.,Mahamid, J. (登録日: 2021-07-18, 公開日: 2022-05-25, 最終更新日: 2022-10-19)

主引用文献

Xue, L.,Lenz, S.,Zimmermann-Kogadeeva, M.,Tegunov, D.,Cramer, P.,Bork, P.,Rappsilber, J.,Mahamid, J.
Visualizing translation dynamics at atomic detail inside a bacterial cell.
Nature, 610:205-211, 2022

PubMed Abstract: Translation is the fundamental process of protein synthesis and is catalysed by the ribosome in all living cells. Here we use advances in cryo-electron tomography and sub-tomogram analysis to visualize the structural dynamics of translation inside the bacterium Mycoplasma pneumoniae. To interpret the functional states in detail, we first obtain a high-resolution in-cell average map of all translating ribosomes and build an atomic model for the M. pneumoniae ribosome that reveals distinct extensions of ribosomal proteins. Classification then resolves 13 ribosome states that differ in their conformation and composition. These recapitulate major states that were previously resolved in vitro, and reflect intermediates during active translation. On the basis of these states, we animate translation elongation inside native cells and show how antibiotics reshape the cellular translation landscapes. During translation elongation, ribosomes often assemble in defined three-dimensional arrangements to form polysomes. By mapping the intracellular organization of translating ribosomes, we show that their association into polysomes involves a local coordination mechanism that is mediated by the ribosomal protein L9. We propose that an extended conformation of L9 within polysomes mitigates collisions to facilitate translation fidelity. Our work thus demonstrates the feasibility of visualizing molecular processes at atomic detail inside cells.

PubMed: 36171285
DOI: 10.1038/s41586-022-05255-2

実験手法

ELECTRON MICROSCOPY (3.5 Å)