7OZN

RNA Polymerase II dimer (Class 1)

7OZN の概要

• エントリーDOI: 10.2210/pdb7ozn/pdb
• 関連するPDBエントリー: 7OZO 7OZP
• EMDBエントリー: 13129 13130 13131 13132
• 分子名称: RPB1, RPB10, RNA_pol_L_2 domain-containing protein, ... (13 entities in total)
• 機能のキーワード: polymerase, dimer, complex, transcription
• 由来する生物種: Sus scrofa domesticus (domestic pig); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 1035423.24

構造登録者

Aibara, S.,Dienemann, C.,Cramer, P. (登録日: 2021-06-28, 公開日: 2021-10-06, 最終更新日: 2024-07-17)

主引用文献

Aibara, S.,Dienemann, C.,Cramer, P.
Structure of an inactive RNA polymerase II dimer.
Nucleic Acids Res., 49:10747-10755, 2021

PubMed Abstract: Eukaryotic gene transcription is carried out by three RNA polymerases: Pol I, Pol II and Pol III. Although it has long been known that Pol I can form homodimers, it is unclear whether and how the two other RNA polymerases dimerize. Here we present the cryo-electron microscopy (cryo-EM) structure of a mammalian Pol II dimer at 3.5 Å resolution. The structure differs from the Pol I dimer and reveals that one Pol II copy uses its RPB4-RPB7 stalk to penetrate the active centre cleft of the other copy, and vice versa, giving rise to a molecular handshake. The polymerase clamp domain is displaced and mobile, and the RPB7 oligonucleotide-binding fold mimics the DNA-RNA hybrid that occupies the cleft during active transcription. The Pol II dimer is incompatible with nucleic acid binding as required for transcription and may represent an inactive storage form of the polymerase.

PubMed: 34530439
DOI: 10.1093/nar/gkab783

実験手法

ELECTRON MICROSCOPY (3.5 Å)