7OWX

Structure of coiled-coil tetramer from SARS-CoV-2 spike stalk region

7OWX の概要

• エントリーDOI: 10.2210/pdb7owx/pdb
• 分子名称: Spike protein S2, 1,2-ETHANEDIOL, ZINC ION, ... (4 entities in total)
• 機能のキーワード: spike, sars-cov-2, coiled-coil, tetramer, virus, coronavirus, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 13191.39

構造登録者

Zivic, Z.,Hadzi, S. (登録日: 2021-06-21, 公開日: 2022-03-16, 最終更新日: 2024-06-19)

主引用文献

Zivic, Z.,Strmsek, Z.,Novinec, M.,Lah, J.,Hadzi, S.
Structural polymorphism of coiled-coils from the stalk domain of SARS-CoV-2 spike protein.
Faseb J., 36:e22199-e22199, 2022

PubMed Abstract: Spike trimer plays a key role in SARS-CoV-2 infection and vaccine development. It consists of a globular head and a flexible stalk domain that anchors the protein into the viral membrane. While the head domain has been extensively studied, the properties of the adjoining stalk are poorly understood. Here, we characterize the coiled-coil formation and thermodynamic stability of the stalk domain and its segments. We find that the N-terminal segment of the stalk does not form coiled-coils and remains disordered in solution. The C-terminal stalk segment forms a trimeric coiled-coil in solution, which becomes significantly stabilized in the context of the full-length stalk. Its crystal structure reveals a novel antiparallel tetramer coiled-coil with an unusual combination of a-d and e-a-d hydrophobic core packing. Structural analysis shows that a subset of hydrophobic residues stabilizes different coiled-coil structures: trimer, tetramer, and heterohexamer, underscoring a highly polymorphic nature of the SARS-CoV-2 stalk sequence.

PubMed: 35157347
DOI: 10.1096/fj.202101670R

実験手法

X-RAY DIFFRACTION (1.929 Å)